Occurrence of free N-glycans with a single GlcNAc at the reducing termini in animal sera
Occurrence of free N-glycans with a single GlcNAc at the reducing termini in animal sera
复制标题
动物血清中还原末端存在带有单一 GlcNAc 的游离 N-聚糖
DOI：
10.1093/glycob/cwab124
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发表时间：
2021
期刊：
影响因子：
4.3
通讯作者：
Suzuki Tadashi
中科院分区：
文献类型：
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作者：
Huang Chengcheng;Seino Junichi;Fujihira Haruhiko;Sato Keiko;Fujinawa Reiko;Sumer-Bayraktar Zeynep;Ishii Nozomi;Matsuo Ichiro;Nakaya Shuichi;Suzuki Tadashi
最近的研究表明，唾液酸游离N-聚糖（FNG）的发生在血清中的各种动物。与胞内FNG主要在其还原末端携带单个N-乙酰葡糖胺（Gn 1-type）不同，这些胞外FNG在其还原末端具有N，N′-二乙酰壳二糖（Gn 2-type）。然而，它们如何形成的详细机制仍不清楚。在这项研究中，我们报告了一种改进的方法从血清中分离FNGs，发现，不仅唾液酸FNGs，而且中性FNGs存在于动物血清中。大多数中性寡甘露糖型FNG被发现是Gn 1型。我们还发现一小部分唾液酸FNG是Gn 1型。Gn 1型唾液酸FNG的比例在物种之间变化，并且似乎与溶酶体壳聚糖酶活性的分布部分相关。我们还鉴定了类似于牛奶低聚糖的小唾液酸化聚糖，如血清中的唾液酸乳糖或唾液酸N-乙酰乳糖胺。我们的研究结果表明，血清中有各种各样的游离寡糖，其形成机制比目前设想的要复杂得多。
Recent studies demonstrated the occurrence of sialyl freeN-glycans (FNGs) in sera from a variety of animals. Unlike the intracellular FNGs that mainly carry a singleN-acetylglucosamine at their reducing termini (Gn1-type), these extracellular FNGs have anN,N′-diacetylchitobiose at their reducing termini (Gn2-type). The detailed mechanism for how they are formed, however, remains unclarified. In this study, we report on an improved method for isolating FNGs from sera and found that, not only sialyl FNGs, but also neutral FNGs are present in animal sera. Most of the neutral oligomannose-type FNGs were found to be Gn1-type. We also found that a small portion of sialyl FNGs were Gn1-type. The ratio of Gn1-type sialyl FNGs varies between species, and appears to be partially correlated with the distribution of lysosomal chitobiase activity. We also identified small sialylated glycans similar to milk oligosaccharides, such as sialyl lactose or sialylN-acetyllactosamine in sera. Our results indicate that there are varieties of free oligosaccharides in sera and the mechanism responsible for their formation is more complicated than currently envisaged.