Distribution of high density lipoprotein particles with different apoprotein composition: particles with A-I and A-II and particles with A-I but no A-II.
Distribution of high density lipoprotein particles with different apoprotein composition: particles with A-I and A-II and particles with A-I but no A-II.
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发表时间：
1982-07
影响因子：
6.5
通讯作者：
M. Cheung;J. Albers
中科院分区：
文献类型：
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作者：
M. Cheung;J. Albers
从两名男性和两名女性中分离出d 1.063-1.21 g/ml的高密度脂蛋白，通过平衡CsCl梯度离心分离高密度脂蛋白（HDL）。分析了各种HDL亚组分的载脂蛋白（apo） A-I, A-II， B， D和E以及主要脂质含量。ApoA-I和A-II在整个密度梯度中均存在，最大浓度在1.105 ~ 1.120 g/ml之间。ApoE在HDL各组分中均存在，且低密度组分含量较高。相反，apoD的浓度随着HDL分数密度的增加而增加。每个密度亚段用抗a - i和抗a - ii免疫球蛋白定量沉淀。基本上，所有密度亚组中的所有A-II都是用免疫球蛋白沉淀的。用抗A-II免疫球蛋白沉淀的每个密度亚段的颗粒的A-I/A-II摩尔比约为2.0（范围为1.9-2.3）。然而，用抗A-I免疫球蛋白沉淀的颗粒的A-I/A-II摩尔比与亚组分的A-I/A-II摩尔比相同（范围为2.1-7.1）。A-I/A-II摩尔比约为2的亚组分（d 1.105 ~ 1.149 g/ml）在含A-I但不含A-II的颗粒中，A-I的比例最小。相反，在A-I/A-II摩尔比最高的亚组分（d 1.063 ~ 1.075 g/ml）中，含A-I而不含A-II的颗粒中apoA-I的比例最大。这些数据表明HDL至少包含两种类型的颗粒：a - i和a - ii的摩尔比为2:1的颗粒，以及含有a - i但不含a - ii的颗粒。在不同HDL密度亚组中观察到的A-I/A-II比值的变化是由于这两种类型的颗粒的不同比例。
High density lipoproteins (HDL) were subfractionated by equilibrium CsCl gradient centrifugation of the d 1.063-1.21 g/ml HDL fraction isolated from two men and two women. The various HDL subfractions were analyzed for their apoproteins (apo) A-I, A-II, B, D, and E and the major lipid contents. ApoA-I and A-II were found throughout the density gradient with the maximum concentration between the d 1.105 and 1.120 g/ml fractions. ApoE was found in all HDL fractions with the higher concentration in the lower density fractions. Conversely, the concentration of apoD increased as the density of the HDL fraction increased. Each density subfraction underwent quantitative precipitation with anti-A-I and anti-A-II immunoglobulin. Essentially all A-II in all density subfractions was precipitated with either immunoglobulin. Particles from each density subfraction precipitated with anti-A-II immunoglobulin had an A-I/A-II molar ratio of approximately 2.0 (range 1.9-2.3). However, particles precipitated with anti-A-I immunoglobulin had A-I/A-II molar ratios identical to the A-I/A-II ratio of the subfraction (range 2.1-7.1). The subfractions (d 1.105-1.149 g/ml fractions) with A-I/A-II molar ratios of about 2 had the least proportion of A-I in particles containing A-I but not A-II. Conversely, the subfractions (d 1.063-1.075 g/ml fractions) with the highest A-I/A-II molar ratio had the greatest proportion of apoA-I in particles containing A-I but not A-II. These data indicate that HDL contains at least two types of particles: particles with both A-I and A-II in a 2:1 molar ratio, and particles containing A-I but no A-II. The variation in A-I/A-II ratio observed in different HDL density subfractions was due to the different proportions of these two types of particles.