Measurement of immunoglobulin M (IgM), IgG, and IgA antibodies against Yersinia enterocolitica by enzyme-linked immunosorbent assay: persistence of serum antibodies during disease
Measurement of immunoglobulin M (IgM), IgG, and IgA antibodies against Yersinia enterocolitica by enzyme-linked immunosorbent assay: persistence of serum antibodies during disease
复制标题
通过酶联免疫吸附试验测量抗小肠结肠炎耶尔森菌的免疫球蛋白 M (IgM)、IgG 和 IgA 抗体：疾病期间血清抗体的持续存在
DOI：
--
复制
发表时间：
1979
影响因子：
9.4
通讯作者：
K. Granfors
中科院分区：
文献类型：
--
作者：
K. Granfors
本文描述了一种用于检测和定量人免疫球蛋白M（IgM）、IgG和伊加抗小肠结肠炎耶尔森氏菌抗体的酶联免疫吸附试验。将福尔马林化或热处理的细菌吸附到专门设计的微量比色皿上，并允许抗体附着到抗原包被的比色皿上。使兔抗人μ、抗人γ和抗人α抗血清与人抗体反应，并通过碱性磷酸酶标记的猪抗兔IgG检测这些类别特异性抗免疫球蛋白。共检测了423份血清。将酶联免疫吸附试验的结果与常规试管凝集试验的结果进行了比较。在6例患者中研究了不同抗体的持久性。IgM类抗体在疾病发作后仅持续1至3个月;因此，在单个样本中出现IgM类耶尔森氏菌抗体表明最近获得的感染。IgG和IgA类抗体的持久性是可变的，并且彼此不平行。值得注意的是，所有三名患者的疾病并发关节炎有IgA类耶尔森氏菌抗体在9至14个月的随访期结束时，在那些没有关节炎的IgA类抗体消失后3个月内发病。
An enzyme-linked immunosorbent assay for the detection and quantitation of human immunoglobulin M (IgM), IgG, and IgA antibodies against Yersinia enterocolitica is described. Formalinized or heat-treated bacteria were adsorbed onto specially designed microcuvettes, and antibodies were allowed to attach to the antigen-coated cuvettes. Rabbit anti-human mu, anti-human gamma, and anti-human alpha antisera were allowed to react with human antibodies, and these class-specific anti-immunoglobulins were detected by alkaline phosphatase-labeled swine anti-rabbit IgG. A total of 423 sera were tested. The results obtained with the enzyme-linked immunosorbent assay were compared with the results of the conventional tube agglutination test. Persistence of different antibodies was studied in six patients. Antibodies of the IgM class persisted only for 1 to 3 months after onset of the disease; thus the occurence of IgM-class Yersinia antibodies in a single sample indicates a recently acquired infection. The persistence of the IgG- and IgA-class antibodies was variable and not parallel with each other. Remarkably, all three patients in which the disease was complicated with arthritis had IgA-class Yersinia antibodies at the end of the follow-up period of 9 to 14 months, and in those without arthritis the IgA-class antibodies disappeared within 3 months after onset of the disease.