On the cyto-toxicity caused by quantum dots
On the cyto-toxicity caused by quantum dots
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DOI：
10.1111/j.1348-0421.2004.tb03478.x
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发表时间：
2004-01-01
影响因子：
2.6
通讯作者：
Yamamoto, K
中科院分区：
文献类型：
--
作者：
Shiohara, A;Hoshino, A;Yamamoto, K
量子点（QD）如CdSe QD已被引入作为新的荧光团。与抗体缀合的量子点开始广泛用于免疫染色。然而，在文献中对量子点的毒性仍然没有足够的分析。因此，我们评估了量子点对生物学应用造成的细胞损伤。我们进行细胞活力测定以确定取决于巯基十一烷酸（MUA）QD的大小和颜色以及细胞类型的细胞损伤的差异。结果表明，随着MUA-QDs浓度的增加，细胞活力下降。而在具有红色荧光QD（QD 640）的Vero细胞（非洲绿色猴肾细胞）中，细胞损伤较其他细胞小。此外，通过流式细胞术测定，我们发现由MUA-QD引起的这种细胞损伤在孵育4-6小时后变成细胞死亡。从上述两种测定中，我们发现存在一定浓度范围的MUA-QD，其中细胞活力降低而不发生细胞死亡，因此我们得出结论，当将MUA-QD应用于活生物体时，即使在低浓度下也应该给予注意。
Quantum dots (QDs) such as CdSe QDs have been introduced as new fluorophores. The QDs conjugated with antibody are starting to be widely used for immunostaining. However there is still not sufficient analysis of the toxicity of QDs in the literature. Therefore we evaluated the cell damage caused by the quantum dots for biological applications. We performed cell viability assay to determine the difference in cell damage depending on the sizes and colors of mercapto-undecanoic acid (MUA) QDs and the cell types. The results showed that the cell viability decreased with increasing concentration of MUA-QDs. But in the case of Vero cell (African green monkey's kidney cell) with red fluorescence QD (QD640), the cell damage was less than for the others. Furthermore through the flow cytometry assay we found that this cell damage caused by MUA-QD turned out to be cell death after 4-6-hr incubation. From the two assays described above, we found that there is a range of concentration of MUA-QDs where the cell viability decreased without cell death occurring and thus we conclude that attention should be given when MUA-QDs are applied to living organisms even in low concentrations.