Reliable cell cycle commitment in budding yeast is ensured by signal integration.
Reliable cell cycle commitment in budding yeast is ensured by signal integration.
复制标题
信号整合确保出芽酵母可靠的细胞周期承诺
作者：
Liu X;Wang X;Yang X;Liu S;Jiang L;Qu Y;Hu L;Ouyang Q;Tang C
细胞命运决定对生命至关重要，但当信号嘈杂且随时间波动时，它们的可靠性如何实现却知之甚少。在这项研究中，我们表明，在芽殖酵母，决定细胞周期的承诺（开始）是由其触发信号Cln 3的时间整合。我们进一步确定启动阻遏物Whi 5作为整合子。Cln 3-Cdk 1的瞬时激酶活性随时间在磷酸化的Whi 5上记录，并且仅当磷酸化的Whi 5达到阈值时才做出决定。在不同的营养条件下，细胞通过调节Whi 5浓度来调节阈值，以协调生长和分裂。我们的工作表明，以前在动物决策行为中发现的信号整合策略，在细胞水平上被采用，以减少噪音和最小化不确定性。DOI：http://dx.doi.org/10.7554/eLife.03977.001网站
Cell fate decisions are critical for life, yet little is known about how their reliability is achieved when signals are noisy and fluctuating with time. In this study, we show that in budding yeast, the decision of cell cycle commitment (Start) is determined by the time integration of its triggering signal Cln3. We further identify the Start repressor, Whi5, as the integrator. The instantaneous kinase activity of Cln3-Cdk1 is recorded over time on the phosphorylated Whi5, and the decision is made only when phosphorylated Whi5 reaches a threshold. Cells adjust the threshold by modulating Whi5 concentration in different nutrient conditions to coordinate growth and division. Our work shows that the strategy of signal integration, which was previously found in decision-making behaviors of animals, is adopted at the cellular level to reduce noise and minimize uncertainty. DOI: http://dx.doi.org/10.7554/eLife.03977.001