Effects of ovariectomy and intrinsic aerobic capacity on tissue-specific insulin sensitivity.
Effects of ovariectomy and intrinsic aerobic capacity on tissue-specific insulin sensitivity.
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卵巢切除术和内在有氧能力对组织特异性胰岛素敏感性的影响。
DOI：
10.1152/ajpendo.00434.2015
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Vieira-Potter,VictoriaJ
中科院分区：
文献类型：
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作者：
Park,Young-Min;Rector,RScott;Thyfault,JohnP;Zidon,TereseM;Padilla,Jaume;Welly,RebeccaJ;Meers,GraceM;Morris,MatthewE;Britton,StevenL;Koch,LaurenG;Booth,FrankW;Kanaley,JillA;Vieira-Potter,VictoriaJ
与低容量运行（LCR）大鼠相比，高容量运行（HCR）大鼠在卵巢切除术（OVX）诱导的胰岛素抵抗（IR）的早期（即术后~ 11周）得到保护。本研究的目的是利用高胰岛素正糖钳来确定1)当OVX后的时间延长至27周时，HCR大鼠是否仍然对OVX诱导的IR有保护作用，2)组织特异性葡萄糖摄取差异负责久坐条件下HCR大鼠的保护。雌性HCR和LCR大鼠（n= 40，年龄~ 22周）随机接受OVX或sham （SHM）手术，然后在手术后27周进行夹钳。[3-3H]葡萄糖用于测定葡萄糖清除率，而2-[14C]脱氧葡萄糖（2- dg）用于评估骨骼肌、棕色脂肪组织（BAT）、皮下白色脂肪组织（WAT）和内脏WAT中的葡萄糖摄取。OVX降低了两组的葡萄糖输注率和葡萄糖清除率，但HCR的胰岛素敏感性优于LCR （P< 0.05）。在这两组中，OVX显著降低了比目鱼肌和腓肠肌的葡萄糖摄取；然而，与LCR相比，HCR的腓肠肌葡萄糖摄取增加了约40% （P< 0.05）。与LCR相比，HCR在BAT和内脏WAT中也表现出更大的葡萄糖摄取（P< 0.05），但这两种组织均未受到OVX的影响。总之，OVX损害HCR和LCR大鼠的胰岛素敏感性，可能是由胰岛素介导的骨骼肌葡萄糖摄取受损驱动的。HCR大鼠有更大的骨骼肌、BAT和WAT胰岛素介导的葡萄糖摄取，这可能有助于防止ovx相关的胰岛素抵抗。
High-capacity running (HCR) rats are protected against the early (i.e., ∼11 wk postsurgery) development of ovariectomy (OVX)-induced insulin resistance (IR) compared with low-capacity running (LCR) rats. The purpose of this study was to utilize the hyperinsulinemic euglycemic clamp to determine whether1) HCR rats remain protected from OVX-induced IR when the time following OVX is extended to 27 wk and2) tissue-specific glucose uptake differences are responsible for the protection in HCR rats under sedentary conditions. Female HCR and LCR rats (n= 40; aged ∼22 wk) randomly received either OVX or sham (SHM) surgeries and then underwent the clamp 27 wk following surgeries. [3-3H]glucose was used to determine glucose clearance, whereas 2-[14C]deoxyglucose (2-DG) was used to assess glucose uptake in skeletal muscle, brown adipose tissue (BAT), subcutaneous white adipose tissue (WAT), and visceral WAT. OVX decreased the glucose infusion rate and glucose clearance in both lines, but HCR had better insulin sensitivity than LCR (P< 0.05). In both lines, OVX significantly reduced glucose uptake in soleus and gastrocnemius muscles; however, HCR showed ∼40% greater gastrocnemius glucose uptake compared with LCR (P< 0.05). HCR also exhibited greater glucose uptake in BAT and visceral WAT compared with LCR (P< 0.05), yet these tissues were not affected by OVX in either line. In conclusion, OVX impairs insulin sensitivity in both HCR and LCR rats, likely driven by impairments in insulin-mediated skeletal muscle glucose uptake. HCR rats have greater skeletal muscle, BAT, and WAT insulin-mediated glucose uptake, which may aid in protection against OVX-associated insulin resistance.