UNCOUPLING CELL FATE DETERMINATION FROM PATTERNED CELL-DIVISION IN THE DROSOPHILA EYE
UNCOUPLING CELL FATE DETERMINATION FROM PATTERNED CELL-DIVISION IN THE DROSOPHILA EYE
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DOI：
10.1126/science.270.5238.983
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发表时间：
1995-11-10
期刊：
影响因子：
56.9
通讯作者：
HARIHARAN, IK
中科院分区：
文献类型：
--
作者：
DENOOIJ, JC;HARIHARAN, IK
在发育中的果蝇眼睛中，细胞增殖和细胞命运规范受到严格的时空控制。从五细胞前簇中排除的细胞同步进入一个额外的细胞周期，即第二个有丝分裂波，之后依次招募剩余的细胞。当第二次有丝分裂波被人类细胞周期蛋白依赖性激酶抑制剂p21（CIP1/WAF1）阻断时，每种细胞类型仍然被指定。因此，细胞命运决定的调节独立于前体细胞的分裂模式。然而，需要第二次有丝分裂波来产生适当数量的每种细胞类型。此外，p21 可以阻止前体细胞增殖并允许在体内进行适当的命运选择。
Cell proliferation and cell fate specification are under strict spatiotemporal control in the developing Drosophila eye. Cells excluded from five-cell preclusters synchronously enter a single additional cell cycle, the second mitotic wave, after which the remaining cells are sequentially recruited. When the second mitotic wave was blocked with the human cyclin-dependent kinase inhibitor p21(CIP1/WAF1), each cell type was still specified. Hence, cell fate determination is regulated independently of the division pattern of precursor cells. However, the second mitotic wave is needed to generate appropriate numbers of each cell type. Moreover, p21 can arrest precursor cell proliferation and allow appropriate fate choice in vivo.