Rapid degeneration of Drosophila olfactory neurons in Orco mutant maxillary palps.
Rapid degeneration of Drosophila olfactory neurons in Orco mutant maxillary palps.
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DOI：
10.17912/micropub.biology.000398
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发表时间：
2021-05-14
影响因子：
--
通讯作者：
Potter CJ
中科院分区：
文献类型：
--
作者：
Task D;Potter CJ
果蝇醋蝇有两个嗅觉器官：触角和上颌须。这些组织中的嗅觉神经元通过气味受体对气味做出反应。昆虫气味受体是两种蛋白质的异四聚体：气味结合 OrX 亚基和气味受体辅助受体 (Orco)。 Orco 的突变会破坏气味受体的形成，并消除嗅觉反应。 Orco 突变体中的一些触角嗅觉神经元先前已被证明会退化。在这里，我们检查 Orco 突变体中上颌须嗅神经元是否也退化。我们发现 Orco 突变型上颌须嗅觉神经元的退化发生得比触角报道的更广泛、更迅速，在羽化后 7 天，约 60% 的突变嗅觉神经元在上颌须中消失。有趣的是，表达 Or42a 受体的 Orco 突变嗅觉神经元子集似乎能够抵抗变性。这些结果表明上颌触肢可能是检查感觉神经元神经变性分子机制的合适模型。
Drosophila melanogaster vinegar flies have two olfactory organs: the antenna and maxillary palp. Olfactory neurons in these tissues respond to odorants via odorant receptors. Insect odorant receptors are heterotetramers of two proteins: an odorant binding OrX subunit and an Odorant Receptor Co-Receptor (Orco). Mutation of Orco disrupts odorant receptor formation, and abolishes olfactory responses. Some antennal olfactory neurons in Orco mutants have been previously shown to degenerate. Here, we examine if maxillary palp olfactory neurons also degenerate in Orco mutants. We find degeneration occurs both more broadly and more rapidly in Orco mutant maxillary palp olfactory neurons than reported for antennae, with ~60% of all mutant olfactory neurons absent in maxillary palps by 7 days post eclosion. Interestingly, the subset of Orco mutant olfactory neurons that express the Or42a receptor appear resistant to degeneration. These results suggest the maxillary palp might be a suitable model for examining the molecular mechanisms underlying neurodegeneration in sensory neurons.