In vivo photoacoustic tumor tomography using a quinoline-annulated porphyrin as NIR molecular contrast agent.
In vivo photoacoustic tumor tomography using a quinoline-annulated porphyrin as NIR molecular contrast agent.
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DOI：
10.1039/c6ob02640k
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发表时间：
2017-01-25
影响因子：
3.2
通讯作者：
Brückner C
中科院分区：
文献类型：
--
作者：
Luciano M;Erfanzadeh M;Zhou F;Zhu H;Bornhütter T;Röder B;Zhu Q;Brückner C
本文报道了一种四聚乙二醇修饰的、水溶性喹啉环卟啉的合成及其物理性质。我们以前证明了喹啉环卟啉作为体外近红外光声成像（派）造影剂的能力。喹啉环卟啉衍生物在血清中的溶解度现在允许评估聚乙二醇化衍生物作为小鼠模型中植入肿瘤的派的体内近红外造影剂的功效。当与基准染料ICG相比时，可以显示出多倍的对比度增强，这一发现可以追溯到其电子物理性质（短的三重态寿命、低荧光和单线态氧敏化量子产率）。可以使用790 nm的NIR激发波长，充分利用组织的光学窗口。观察到染料的快速肾清除。它的直接合成，光学性质与进一步的光学微调的可能性，无毒，有利的消除率，和对比度增强，使之成为一个有前途的派造影剂。还证明了使用简单和通用的缀合策略将派发色团与荧光标签缀合的能力。
The synthesis and photophysical properties of a tetra-PEG-modified and freely water-soluble quinoline-annulated porphyrin are described. We previously demonstrated the ability of quinoline-annulated porphyrins to act as an in vitro NIR photoacoustic imaging (PAI) contrast agent. The solubility of the quinoline-annulated porphyrin derivative in serum now allowed the assessment of the efficacy of the PEGylated derivative as an in vivo NIR contrast agent for the PAI of an implanted tumor in a mouse model. A multi-fold contrast enhancement when compared to the benchmark dye ICG could be shown, a finding that could be traced to its photophysical properties (short triplet lifetimes, low fluorescence and singlet oxygen sensitization quantum yields). A NIR excitation wavelength of 790 nm could be used, fully taking advantage of the optical window of tissue. Rapid renal clearance of the dye was observed. Its straight-forward synthesis, optical properties with the possibility for further optical fine-tuning, nontoxicity, favorable elimination rates, and contrast enhancement make this a promising PAI contrast agent. The ability to conjugate the PAI chromophore with a fluorescent tag using a facile and general conjugation strategy was also demonstrated.