Diagnostic value of MUC4 immunostaining in distinguishing epithelial mesothelioma and lung adenocarcinoma
Diagnostic value of MUC4 immunostaining in distinguishing epithelial mesothelioma and lung adenocarcinoma
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DOI：
10.1038/modpathol.3800027
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发表时间：
2004-02-01
期刊：
影响因子：
7.5
通讯作者：
Copin, MC
中科院分区：
文献类型：
--
作者：
Llinares, K;Escande, F;Copin, MC
胸膜恶性间皮瘤和胸膜浸润腺癌之间的区别有复杂的治疗和法医学意义。尽管腺癌相关抗体和一个或两个间皮瘤标记物的面板在此目的是有用的，大多数这些抗体不是完全特异性的。我们确定了MUC 4免疫染色在这个问题上的诊断价值。原位杂交和RT-PCR检测MUC 4基因表达。MUC 4是一种膜结合粘蛋白，已被认为与人类和大鼠的恶性进展有关。MUC 4基因在内胚层来源的各种正常上皮组织和癌中表达。在呼吸道中，MUC 4转录物已在正常呼吸道上皮和肺癌中检测到。35例肺腺癌石蜡包埋组织中32例（91.4%）表达MUC 4蛋白。41例恶性间皮瘤和32例良性间皮瘤细胞均无MUC 4蛋白和mRNA表达。我们的结论是，MUC 4是一个非常具体的（100%）和敏感性（91.4%）的肺腺癌的石蜡包埋组织，可能是有用的诊断实践中的恶性间皮瘤和腺癌之间的区别标记。
The distinction between pleural malignant mesothelioma and pleural infiltration by adenocarcinomas has complex therapeutic and medicolegal implications. Although the panel of adenocarcinoma-associated antibodies and one or two mesothelioma markers is useful in this purpose, most of these antibodies are not totally specific. We determined the diagnostic value of MUC4 immunostaining in this issue. MUC4 gene expression was also studied by in situ hybridization and RT-PCR. MUC4 is a membrane-bound mucin that has been suggested to be implicated in malignant progression in humans and rats. The MUC4 gene is expressed in various normal epithelial tissues of endodermic origin and carcinomas. In the respiratory tract, MUC4 transcripts have been detected in normal respiratory epithelium and lung carcinomas. MUC4 protein was expressed in 32 of 35 (91.4%) lung adenocarcinomas on paraffin-embedded tissue. None of the 41 malignant mesotheliomas nor the 32 cases of benign mesothelial cells expressed MUC4 at the protein and mRNA levels. We conclude that MUC4 is a very specific (100%) and sensitive (91.4%) marker of lung adenocarcinomas on paraffin-embedded tissue that could be useful in diagnostic practice in the distinction between malignant mesothelioma and adenocarcinoma.