Berries as a potential transmission vehicle for taeniid eggs
Berries as a potential transmission vehicle for taeniid eggs
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DOI：
10.1016/j.parint.2019.01.008
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发表时间：
2019-06-01
影响因子：
1.9
通讯作者：
Sukura, Antti
中科院分区：
文献类型：
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作者：
Malkamaki, Sanna;Nareaho, Anu;Sukura, Antti
以非人畜共患病的阔颈带绦虫卵为模型，研究了野生森林浆果作为带绦虫卵传播媒介的潜在作用。所研究的浆果是越橘（欧洲越橘）（1 m（2）小区，n = 10）和越橘（欧洲越橘）（1 m（2）小区，n = 11）。在经营林内各小区用3万或6万T.将侧颈蛙卵悬浮在水中，并在喷雾后24小时收集浆果。用水冲洗浆果，并将水通过1 mm和63 μ m的筛子筛分以除去粗物质，并通过20 μ m的筛子收集可能的卵。在用荧光Calcofluor白色染色剂处理后，通过显微镜检查一小部分筛分的材料，所述荧光Calcofluor染色剂与蛋壳几丁质结合。在人工加标样品的回收试验中，检测限为100 g商业冷冻越橘和越橘中5个卵。在所有10个实验污染的越橘样品和11个越橘样品中的10个中检测到绦虫卵。还分析了筛分碎片中的T。用半定量PCR方法对侧颈藻DNA进行检测。所有样品在使用T.扩增线粒体NADH脱氢酶亚基1基因的短片段。这表明灌木中被污染的森林浆果含有T。这些浆果可作为绦虫卵载体，并可能对人类构成危险。
Potential role of wild forest berries as a transmission vehicle for taeniid eggs was examined using non-zoonotic Taenia laticollis eggs as a model. The berries studied were bilberries (Vaccinium myrtillus) (1 m(2) plot, n = 10) and lingonberries (Vaccinium vitis-idaea) (1 m(2) plot, n = 11). The plots in the managed forest were evenly sprayed with 30,000 or 60,000 T. laticollis eggs suspended in water, and berries were collected 24 h after spraying. The berries were rinsed with water, and the water was sieved through a 1-mm and a 63-mu m sieve to remove coarse material and through a 20-mu m sieve to collect possible eggs. A small proportion of the sieved material was examined by microscopy after treatment with fluorescent Calcofluor White stain, which binds to eggshell chitin. In the recovery tests in artificially spiked samples, the detection limit was 5 eggs in 100 g of commercial frozen bilberries and lingonberries. Taeniid eggs were detected in all of the 10 experimentally contaminated bilberry samples and in 10 of 11 lingonberry samples. The sieved debris was also analyzed for T. laticollis DNA using semiquantitative PCR. All samples were positive in quantitative SYBR Green real-time PCR using a T. laticollis-specific primer pair amplifying a short fragment of mitochondrial NADH dehydrogenase subunit 1 gene. This indicates that forest berries contaminated in shrubs contained T. laticollis eggs, and that berries can serve as a vehicle for taeniid eggs and may pose a possible risk to humans.