Tickling stimulation causes the up-regulation of the kallikrein family in the submandibular gland of the rat
Tickling stimulation causes the up-regulation of the kallikrein family in the submandibular gland of the rat
复制标题
搔痒刺激导致大鼠颌下腺中激肽释放酶家族的上调
DOI：
10.1016/j.bbr.2012.09.001
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发表时间：
2013
期刊：
影响因子：
2.7
通讯作者：
Urayama O
中科院分区：
文献类型：
--
作者：
Yamamuro T;Hori M;Nakagawa Y;Hayashi T;Sakamoto S;Ohnishi J;Takeuchi S;Mihara Y;Shiga T;Murakami K;Urayama O
我们最近发现，伴随着积极情绪的触觉刺激（挠痒痒）改变了大鼠下丘脑中许多基因的表达（Hori et al., 2009[15]）。本研究研究了反复挠痒对大鼠唾液腺基因表达的影响。DNA微阵列分析显示，刺激4周后，klikrein （Klk）家族的几个基因显著上调，α -淀粉酶（淀粉酶）基因下调。对挠痒2周的大鼠颌下腺进行实时PCR定量分析，Klk基因家族18个成员的Klk1、Klk2 （Klk1c2、Tonin）、Klk7 （Klk1l）、Klk1b3（神经生长因子，γ）、Klk1c10、Klks3 （Klk1c9）和GK11 mrna与轻触和未触碰的对照组相比显著升高2 - 5倍，下颌淀粉酶降低。免疫印迹分析显示，在颌下腺全细胞裂解液中Klk7蛋白表达增加。用抗klk7多克隆抗体进行免疫组化分析，与轻触和未触碰的对照组相比，挠痒大鼠颌下腺导管细胞的免疫染色增加。这些结果表明，中枢神经系统的触觉感觉加工可能通过激素和/或自主神经活动影响外周组织的基因表达。下颌下Klks可能是积极情绪状态的生化标记。
We recently showed that tactile stimulation (tickling) accompanied by positive emotion altered the expression of many genes in the rat hypothalamus (Hori et al., 2009 [15]). In this study, the effect of repeated tickling on gene expressions of the rat salivary gland was examined. After 4-week stimulation, several genes of the kallikrein (Klk) family were remarkably up-regulated and the alpha-amylase (amylase) gene was down-regulated in DNA microarray analysis. In quantitative analysis using real-time PCR of the submandibular gland of the rats tickled for 2 weeks, mRNAs of Klk1, Klk2 (Klk1c2, Tonin), Klk7 (Klk1l), Klk1b3 (Nerve growth factor, gamma), Klk1c10, Klks3 (Klk1c9) and GK11 were significantly 2–5-fold increased among 18 members of the Klk gene family examined and the submandibular amylase was decreased compared with the lightly touched and untouched control rats. In immunoblot analysis the increase in Klk7 protein was observed in the whole cell lysate fraction of the submandibular gland. In immunohistochemical analysis with anti-Klk7 polyclonal antibody, the immunostain was increased in duct cells of the submandibular gland of the tickled rat when compared with the lightly touched and untouched control rats. These results suggest that tactile sensory processing in the central nervous system affects the gene expression in the peripheral tissue probably via hormonal and/or autonomic neural activities. Submandibular Klks may be biochemical markers indicating positive emotional states.