Magnetoliposome for alendronate delivery
Magnetoliposome for alendronate delivery
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DOI：
10.1039/c1jm00060h
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发表时间：
2011-01-01
影响因子：
--
通讯作者：
Seksek, Olivier
中科院分区：
文献类型：
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作者：
Benyettou, Farah;Chebbi, Imene;Seksek, Olivier
已开发出脂质体封装，以保护治疗剂免受酶促降解，并减少剂量和毒副作用。然而，药物从脂质囊泡中选择性、靶向和受控释放仍然难以实现。同时，由于其磁性，超顺磁性氧化铁（SPIO）纳米颗粒也被认为是在其表面接枝治疗药物后良好的递送载体。评估两种特性（磁性靶向和药物封装）的组合以递送抗癌药物：阿仑膦酸盐，一种羟亚甲基二膦酸盐分子。在三种人类癌细胞系 MDA-MB231、A431 和 U87-MG 上测试了接枝阿仑膦酸钠的 γ-Fe2O3 纳米晶体（有或没有脂质体封装）、有或没有磁场。测量细胞毒性以及药物内化。虽然使用不同配方的三种细胞系的结果并不相同，但只有将阿仑膦酸钠接枝的 SPIO 包埋到脂质体中并暴露于磁场才能实现有效的 100% 细胞毒性作用。
Encapsulation into liposomes has been developed in order to allow protection of therapeutical agents against enzymatic degradation, and to reduce doses and toxic side effects. Selective, targeted and controlled release of the drug out of the lipid vesicle is still, however, difficult to achieve. Meanwhile, thanks to their magnetic properties, superparamagnetic iron oxide (SPIO) nanoparticles have also been considered as good delivery vehicles after grafting a therapeutic drug on their surface. A combination of both properties (magnetic targeting and drug encapsulation) is evaluated to deliver an anticancer drug : alendronate, an hydroxymethylene bisphosphonate molecule. gamma-Fe2O3 nanocrystals grafted with alendronate were tested with or without liposome encapsulation, with and without magnetic field, on three human cancer cell lines, MDA-MB231, A431 and U87-MG. Cytotoxicity was measured as well as drug internalization. While results were not identical on the three cell lines with the different formulations, an effective 100% cytotoxic effect could only be achieved with alendronate grafted-SPIO entrapped into liposomes and exposed to a magnetic field.