After the double helix -: Rosalind!Franklin's research on Tobacco mosaic virus
After the double helix -: Rosalind!Franklin's research on Tobacco mosaic virus
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DOI：
10.1086/588626
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发表时间：
2008-06-01
期刊：
影响因子：
0.6
通讯作者：
Morgan, Gregory J.
中科院分区：
文献类型：
--
作者：
Creager, Angela N. H.;Morgan, Gregory J.
罗莎琳德·富兰克林最出名的是她丰富的DNA x射线衍射图，为詹姆斯·沃森和弗朗西斯·克里克的双链螺旋模型提供了重要线索。然而，当她离开国王学院的DNA研究工作时，她的科学生涯并没有结束。1953年，富兰克林转到伯贝克学院J.D. Bernal的晶体学实验室，在那里她将注意力转移到病毒的三维结构上，获得了烟草花叶病毒（TMV）的衍射图，其细节和清晰度前所未有。在接下来的五年里，富兰克林在确定TMV的结构方面取得了重大进展，同时与沃森和克里克保持着积极的联系，他们也在研究病毒结构的各个方面。在20世纪50年代期间，TMV研究的发展说明了新兴的分子生物学领域中核酸结构研究与蛋白质和病毒研究之间的联系。它们还揭示了在沃森和克里克的DNA双螺旋结构模型被争论和证实的那些年里，“双螺旋结构竞赛”的主角们是如何继续在个人和专业上互动的。
Rosalind Franklin is best known for her informative X-ray diffraction patterns of DNA that provided vital clues for James Watson and Francis Crick's double-stranded helical model. Her scientific career did not end when she left the DNA work at King's College, however. In 1953 Franklin moved to J.D. Bernal's crystallography laboratory at Birkbeck College, where she shifted her focus to the three-dimensional structure of viruses, obtaining diffraction patterns of Tobacco mosaic virus (TMV) of unprecedented detail and clarity. During the next five years, while making significant headway on the structural determination of TMV, Franklin maintained an active correspondence with both Watson and Crick, who were also studying aspects of virus structure. Developments in TMV research during the 1950s illustrate the connections in the emerging field of molecular biology between structural studies of nucleic acids and of proteins and viruses. They also reveal how the protagonists of the "race for the double helix" continued to interact personally and professionally during the years when Watson and Crick's model for the double-helical structure of DNA was debated and confirmed.