The CORVET tethering complex interacts with the yeast Rab5 homolog Vps21 and is involved in endo-lysosomal biogenesis
The CORVET tethering complex interacts with the yeast Rab5 homolog Vps21 and is involved in endo-lysosomal biogenesis
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DOI：
10.1016/j.devcel.2007.03.006
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发表时间：
2007-05-01
影响因子：
11.8
通讯作者：
Ungermann, Christian
中科院分区：
文献类型：
--
作者：
Peplowska, Karolina;Markgraf, Daniel F.;Ungermann, Christian
囊泡分裂和融合之间的动态平衡在高尔基体，内体，和液泡/溶酶体的细胞器的身份的维持是至关重要的。其中，它依赖于Rab GTP酶和拴系因子，其功能和调节仍不清楚。我们现在表明，高尔基体，内体和液泡之间的运输是由两个同源的拴系复合物，以前确定的HOPS复合物在液泡和一个新的内体拴系（CORVET）复合物，它与拉布GTALOVPS 21相互作用。这两种复合物共有四种C类Vps蛋白：Vps 11，Vps 16，Vps 18和Vps 33。此外，HOPS复合物含有Vps 41/Vam 2和Vam 6，而CORVET复合物具有Vps 41同源物Vps 8和（h）Vam 6同源物Vps 3。引人注目的是，CORVET和HOPS的ESTA可以相互转换，我们确定了两个额外的中间复合物，都由C类核心结合到Vam 6-Vps 8或Vps 3-Vps 41。我们的数据表明，模块组装的拴系复合物定义细胞器的生物合成的内吞途径。
The dynamic equilibrium between vesicle fission and fusion at Golgi, endosome, and vacuole/lysosome is critical for the maintenance of organelle identity. It depends, among others, on Rab GTPases and tethering factors, whose function and regulation are still unclear. We now show that transport among Golgi, endosome, and vacuole is controlled by two homologous tethering complexes, the previously identified HOPS complex at the vacuole and a novel endosomal tethering (CORVET) complex, which interacts with the Rab GTPase Vps21. Both complexes share the four class C Vps proteins: Vps11, Vps16, Vps18, and Vps33. The HOPS complex, in addition, contains Vps41/Vam2 and Vam6, whereas the CORVET complex has the Vps41 homolog Vps8 and the (h)Vam6 homolog Vps3. Strikingly, the CORVET and HOPS complexe can interconvert; we identify two additional intermediate complexes, both consisting of the class C core bound to Vam6-Vps8 or Vps3-Vps41. Our data suggest that modular assembled tethering complexes define organelle biogenesis in the endocytic pathway.