Characterization and complete genome of the virulent Myoviridae phage JD007 active against a variety of Staphylococcus aureus isolates from different hospitals in Shanghai, China.
Characterization and complete genome of the virulent Myoviridae phage JD007 active against a variety of Staphylococcus aureus isolates from different hospitals in Shanghai, China.
复制标题
对中国上海不同医院分离出的多种金黄色葡萄球菌具有活性的强毒力肌病毒科噬菌体 JD007 的特征和完整基因组
DOI：
10.1186/s12985-017-0701-0
复制
发表时间：
2017-02-08
期刊：
影响因子：
4.8
通讯作者：
Guo X
中科院分区：
文献类型：
--
作者：
Cui Z;Feng T;Gu F;Li Q;Dong K;Zhang Y;Zhu Y;Han L;Qin J;Guo X
随着广泛的耐药细菌的增加，噬菌体治疗的实施正在重新出现。 对金黄色葡萄球菌噬菌体JD 007进行了鉴定和全基因组序列分析。 葡萄球菌噬菌体JD 007被归类为属于肌尾病毒科的基础上，其形态，通过透射电子显微镜观察。其裂解活性在pH 5-11和低于42 °C之间稳定;此外，吸光度曲线显示，在共孵育20分钟后，近90%的病毒颗粒已吸附到其宿主上。完整的基因组大小为141，836 bp，使JD 007成为肌病毒科最大的金黄色葡萄球菌之一。在JD 007基因组中未发现可识别的抗性或毒力基因。JD 007对S.金黄色葡萄球菌分离株，包括上海市不同医院分离的流行株ST 239-MRSA和ST 59-MRSA，抑菌试验表明JD 007对金黄色葡萄球菌有抑制作用。金黄色葡萄球菌生长的感染复数为0.1。结果表明，金黄色葡萄球菌噬菌体JD 007可用于噬菌体治疗或检测金黄色葡萄球菌。金黄色。本文的在线版本（doi：10.1186/s12985-017-0701-0）包含补充材料，可供授权用户使用。
The implementation of phage therapy is re-emerging with the increase in widespread antibiotic-resistant bacteria. Staphylococcus phage JD007 was characterized and its complete genome sequence analysed. Staphylococcus phage JD007 was classified as belonging to the Myoviridae family based on its morphology, as observed by transmission electron microscopy. Its lytic activity was stable between pH 5–11 and below 42 °C; moreover, an absorbance curve showed that nearly 90% of the viral particles had adsorbed to its host after a 20 min co-incubation. The complete genome size is 141,836 bp, making JD007 one of the largest Staphylococcus phages of Myoviridae. No identifiable resistance or virulence genes were found in the JD007 genome. JD007 was able to lyse 95% of S. aureus isolates, including the prevalent ST239-MRSA and ST59-MRSA strains isolated from different hospitals in Shanghai, China, and inhibition assays showed that JD007 could inhibit S. aureus growth at a multiplicity of infection of 0.1. The results suggested that Staphylococcus phage JD007 can potentially be used in phage therapy or for the detection of S. aureus. The online version of this article (doi:10.1186/s12985-017-0701-0) contains supplementary material, which is available to authorized users.