Expression and activity analysis reveal that heme oxygenase (decycling) 1 is associated with blue egg formation.
Expression and activity analysis reveal that heme oxygenase (decycling) 1 is associated with blue egg formation.
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DOI：
10.3382/ps.2010-01143
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发表时间：
2011-04
期刊：
影响因子：
4.4
通讯作者：
Z. P. Wang;R. F. Liu;A. Wang;J. Li;X. Deng
中科院分区：
文献类型：
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作者：
Z. P. Wang;R. F. Liu;A. Wang;J. Li;X. Deng
胆绿素负责蓝色鸡蛋的着色，并由蛋壳腺体分泌到蛋壳上。此前的研究证实，蓝色鸡蛋和棕色鸡蛋的胆绿素含量存在显著差异，但原因尚不清楚。由于该色素是由血红素加氧酶(HO)催化的血红素氧化降解产生的，因此本研究比较了编码HO表达和HO活性的基因Hmox1(Hmox1)在东乡蓝壳鸡(n=12)和东乡褐壳鸡(n=12)壳腺中的差异。结果表明，Hmox1在该基因的表达水平较高(1.58倍；P<0.05)。综上所述，这些结果表明，蓝蛋的形成与Hmox1在东乡蓝壳鸡壳腺中的高表达有关，并表明Hmox1在两组鸡中的差异表达最有可能是由于反式作用因子的改变。
Biliverdin is responsible for the coloration of blue eggs and is secreted onto the eggshell by the shell gland. Previous studies confirmed that a significant difference exists in biliverdin content between blue eggs and brown eggs, although the reasons are still unknown. Because the pigment is derived from oxidative degradation of heme catalyzed by heme oxygenase (HO), this study compared heme oxygenase (decycling) 1 (HMOX1), the gene encoding HO expression and HO activity, in the shell glands of the Dongxiang blue-shelled chicken (n = 12) and the Dongxiang brown-shelled chicken (n = 12). Results showed that HMOX1 was highly expressed at the mRNA (1.58-fold; P 0.05). Taken together, these results show that blue egg formation is associated with high expression of HMOX1 in the shell gland of Dongxiang blue-shelled chickens, and suggest that differential expression of HMOX1 in the 2 groups of chickens is most likely to arise from an alteration in the trans-acting factor.