Small RNAs in tomato fruit and leaf development
Small RNAs in tomato fruit and leaf development
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DOI：
10.1016/j.bbagrm.2007.09.003
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发表时间：
2008-02-01
影响因子：
4.7
通讯作者：
Ding, Biao
中科院分区：
文献类型：
--
作者：
Itaya, Asuka;Bundschuh, Ralf;Ding, Biao
番茄果实和叶片的发育提供了很好的系统来研究不同器官发育的基因调控进化。我们已经分别从番茄果实和叶中鉴定出超过350和700种小RNA。除了保守的microRNA外，90%以上的小RNA是番茄所特有的。我们通过北方杂交证实了一些保守的以及新的推定microRNA的表达。我们的研究结果有助于形成一个基础上的比较研究如何小RNA介导的基因表达，有助于水果和叶子的共同和独特的发育途径的演变。我们已经建立了一个网站（http：//ted.bti.cornwell.edu/digital/sRNA/），以供公众以可搜索的方式访问我们所有的小RNA序列、它们在各自组织中的表达模式以及它们的匹配基因或预测的靶基因。(C)2007 Elsevier B. V.保留所有权利。
Tomato fruit and leaf development offers excellent systems to study the evolution of gene regulation underlying development of different organs. We have identified over 350 and 700 small RNAs from tomato fruit and leaf, respectively. Except for conserved microRNAs, more than 90% of the small RNAs are unique to tomato. We confirmed expression of some conserved as well as novel putative microRNAs by Northern hybridization. Our results help form a basis for comparative studies on how small RNA-mediated gene expression has contributed to the evolution of common and distinct developmental pathways of fruits and leaves. We have established a website (http://ted.bti.cornwell.edu/digital/sRNA/) for public access to all of our small RNA sequences, their expression patterns in respective tissues, and their matching genes or predicted target genes in a searchable manner. (C) 2007 Elsevier B.V. All rights reserved.