HEPARIN AFFINITY OF ANIONIC AND CATIONIC CAPILLARY ENDOTHELIAL CELL-GROWTH FACTORS - ANALYSIS OF HYPOTHALAMUS-DERIVED GROWTH-FACTORS AND FIBROBLAST GROWTH-FACTORS
HEPARIN AFFINITY OF ANIONIC AND CATIONIC CAPILLARY ENDOTHELIAL CELL-GROWTH FACTORS - ANALYSIS OF HYPOTHALAMUS-DERIVED GROWTH-FACTORS AND FIBROBLAST GROWTH-FACTORS
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DOI：
10.1073/pnas.82.3.805
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
SHING, Y
中科院分区：
文献类型：
--
作者：
KLAGSBRUN, M;SHING, Y
牛下丘脑衍生的生长因子（HDGF），垂体成纤维细胞生长因子（FGF）和脑FGF进行了分析，通过色谱固定化肝蛋白和测试的能力，刺激毛细血管内皮细胞（CE）的增殖。在下丘脑中发现了两种不同的CE细胞生长因子，一种是阴离子型（aHDGF;等电点约为5），另一种是阳离子型（cHDGF;等电点约为8）。aHDGF和cHDGF均与固定化肝素紧密粘附。它们分别用0.9-1.1 M NaCl和1.3-1.5 M NaCl洗脱。还发现PGFs和脑FGF与固定化肝素结合并刺激CE细胞增殖。用1.4-1.6 M NaCl洗脱PGEF。脑FGF的洗脱曲线显示，从肝素柱洗脱出CE细胞生长因子活性的2个峰，1个在1.0 M NaCl下，第2个在1.4-1.6 M NaCl下。所有这些生长因子与肝素的紧密结合（特别是aHDGF，其结合是出乎意料的，因为其带负电荷）被呈现为CE细胞生长因子都共享对肝素的亲和力的证据。
Bovine hypothalamus-derived growth factors (HDGF), pituitary fibroblast growth factor (FGF) and brain FGF were analyzed by chromatography on immobilized hepatrin and tested for the ability to stimulate the proliferation of capillary endothelial (CE) cells. Two distinct CE cell growth factors were found in hypothalamus, one anionic (aHDGF; pI [isoelectric point] of about 5) and one cationic (cHDGF; pI of about 8). Both aHDGF and cHDGF adhered tightly to immobilized heparin. They were eluted with 0.9-1.1 M NaCl and 1.3-1.5 M NaCl, respectively. Pituitary and brain FGF were also found to bind to immobilized heparin and to stimulate CE cell proliferation. Pituitary FGF was eluted 1.4-1.6 M NaCl. The elution profile of brain FGF showed that 2 peaks of CE cell growth factor activity were eluted from the heparin column, 1 at 1.0 M NaCl and a 2nd at 1.4-1.6 M NaCl. The tight binding of all of these growth factors to heparin (particularly aHDGF, whose binding is unexpected because of its negative charge) is presented as evidence that CE cell growth factors all share an affinity for heparin.