Impaired cholinergic vasodilation in the cholesterol-fed rabbit in vivo.
Impaired cholinergic vasodilation in the cholesterol-fed rabbit in vivo.
复制标题
胆固醇喂养的兔子体内胆碱能血管舒张受损。
DOI：
10.1007/bf01907027
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发表时间：
1987
影响因子：
9.5
通讯作者：
Henry,PD
中科院分区：
文献类型：
--
作者：
Bossaller,C;Yamamoto,H;Lichtlen,PR;Henry,PD
在动脉粥样硬化的动物和人的大动脉中，体外内皮依赖性胆碱能松弛受到损害。为了评估这种损害的生理意义，我们比较了乙酰胆碱和硝普钠对对照组(n=12)和喂养1%胆固醇饮食10周的兔(n=8)的血管扩张作用。兔后肢经体外循环恒流灌流，灌流压作为总血管阻力的指标，动脉内注射不同浓度的乙酰胆碱(0.01ng0.01~1.000 ng)和硝普钠(0.1ng0.1~1.000 ng)监测后肢血管阻力。在0.1ng硝普钠和1.000 ng硝普钠之间，对照组和胆固醇喂养组的抵抗力变化几乎是相同的。结果表明，在动脉粥样硬化模型中，血管内皮依赖性乙酰胆碱的扩血管反应性降低，而非内皮依赖性硝普钠的作用完全保留。
Endothelium-dependent cholinergic relaxation in vitro is impaired in large arteries of animals and man with atherosclerosis. To assess the physiological importance of this impairment, we compared vasodilator effects of acetylcholine and nitroprusside, an endothelium-independent agent, in control rabbits (n=12) and rabbits fed a 1% cholesterol diet for 10 weeks (n=8). The hindlimb of the rabbits was perfused via an extracorporal loop at a constant flow, and perfusion pressure was used as an index of total vascular resistance.The hindlimb vascular resistance was monitored during intraarterial bolus injections with graded concentrations of acetylcholine (0.01–1.000 ng) and nitroprusside (0.1–1.000 ng) which produced no change in systemic hemodynamics. Between 0.1 and 1.000 ng nitroprusside, resistance changes in control and cholesterol-fed rabbits were virtually identical. In contrast, the resistance change induced by acetylcholine was significantly suppressed in cholesterol-fed rabbits over a wide range of concentrations (1.0–1.000 ng;p<0.05).The results indicate that in a model of atherosclerosis there is a reduced vasodilatory responsiveness to the endothelium-dependent acetylcholine, whereas the effect of the endothelium-independent nitroprusside is fully preserved.