Rational Design of a Stimuli -Responsive Polymer Electrode Interface Coupled with in Vivo Microdialysis for Measurement of Sialic Acid in Live Mouse Brain in Alzheimer′s Disease
Rational Design of a Stimuli -Responsive Polymer Electrode Interface Coupled with in Vivo Microdialysis for Measurement of Sialic Acid in Live Mouse Brain in Alzheimer′s Disease
复制标题
刺激响应聚合物电极接口的合理设计与体内微透析相结合，用于测量阿尔茨海默氏病活体小鼠大脑中的唾液酸
DOI：
10.1021/acssensors.6b00772
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发表时间：
2017-03-01
期刊：
影响因子：
8.9
通讯作者：
Shi, Guoyue
中科院分区：
文献类型：
--
作者：
Ding, Shushu;Cao, Sumei;Shi, Guoyue
灵敏、选择性地监测脑神经系统中唾液酸（SA）的含量，对于研究SA在阿尔茨海默病（AD）病理过程中的作用具有重要意义。在这项工作中，我们首次报道了一种基于新型刺激响应共聚物的电化学生物传感器，用于选择性和灵敏地检测小鼠脑中的SA。值得注意的是，通过协同氢键相互作用，共聚物可以将SA的识别转化为它们的构象转变和润湿性开关，这有助于氧化还原标记物和靶向电极表面的访问和富集，从而显着提高检测灵敏度，检测限低至0.4 pM。除了放大的传感信号，所提出的方法表现出良好的选择性SA相比，潜在的干扰分子共存于复杂的大脑系统，由于苯硼酸（PBA）和SA之间的高亲和力和定向氢键相互作用的共聚物的组合。该电化学生物传感器具有良好的分析性能，并成功地应用于AD小鼠活体脑内SA水平的动态变化研究。首次报道了SA在AD小鼠不同脑区的准确浓度，这有助于我们进一步了解SA在脑生理和病理事件中的作用。
Sensitive and selective monitoring of sialic acid (SA) in cerebral nervous system is of great importance for studying the role that SA plays in the pathological process of Alzheimer's disease (AD). In this work, we first reported an electrochemical biosensor based on a novel stimuli-responsive copolymer for selective and sensitive detection of SA in mouse brain. Notably, through synergetic hydrogen-bonding interactions, the copolymer could translate the recognition of SA into their conformational transition and wettability switch, which facilitated the access and enrichment of redox labels and targets to the electrode surface, thus significantly improving the detection sensitivity with the detection limit down to 0.4 pM. Besides amplified sensing signals, the proposed method exhibited good selectivity toward SA in comparison to potential interference molecules coexisting in the complex brain system due to the combination of high affinity between phenylboronic acid (PBA) and SA and the directional hydrogen-bonding interactions in the copolymer. The electrochemical biosensor with remarkable analytical performance was successfully applied to evaluate the dynamic change of SA level in live mouse brain with AD combined with in vivo midrodialysis. The accurate concentration of SA in different brain regions of live mouse with AD has been reported for the first time, which is beneficial for progressing our understanding of the role that SA plays in physiological and pathological events in the brain.