In vitro digestion combined with cellular assay to determine the antioxidant activity in Chinese bayberry (Myrica rubra Sieb. et Zucc.) fruits: A comparison with traditional methods
In vitro digestion combined with cellular assay to determine the antioxidant activity in Chinese bayberry (Myrica rubra Sieb. et Zucc.) fruits: A comparison with traditional methods
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DOI：
10.1016/j.foodchem.2013.09.071
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发表时间：
2014-03-01
期刊：
影响因子：
8.8
通讯作者：
Ye, Xingqian
中科院分区：
文献类型：
--
作者：
Huang, Haizhi;Sun, Yujing;Ye, Xingqian
传统的化学提取方法（即，提取物）与化学抗氧化活性测定结合不能评估真实的抗氧化活性。体外消化（即，采用细胞抗氧化活性（CAA）法测定杨梅果实的抗氧化活性。结果表明，与浸提液相比，紫茎泽兰的游离酚酸含量较高，而总酚含量和总花色苷含量较低。比较了ABTS、FRAP、DPPH、ORAC和CAA等抗氧化活性测定方法。消化者的ABTS较低。FRAP和DPPH值高于浸提液，CAA值高于浸提液。FPA与化学抗氧化剂测定结果具有较好的相关性。木麻黄总酚含量与CAA值相关性较高（R-2 = 0.96），而提取物的相关性不高（R-2 = 0.58）。CAA和化学测定之间也有较高的相关性。(C)2013爱思唯尔有限公司保留所有权利。
The traditional method of chemical extraction (i.e., extracts), combined with chemical antioxidant activity assays cannot assess the real antioxidant activity. In vitro digestion (i.e., digesta) with a cellular antioxidant activity (CAA) assay was developed for the determination of antioxidant activity in Chinese bayberry fruits. In this study, pretreatment methods were studied and the results showed that digesta had more free phenolic acids (FPA) but less total phenolic content (TPC) and total anthocyanin content (TAC) than extracts. Antioxidant activity assays, including ABTS, FRAP, DPPH, ORAC and CAA, were compared. Digesta had lower ABTS. FRAP and DPPH values but higher CAA values than extracts. FPA were better correlated with the chemical antioxidant assays in digesta. The correlations were high between TPC and CAA values in digesta (R-2 = 0.96) but not extracts (R-2 = 0.58). Higher correlations were also obtained between CAA and chemical assays in digesta. (C) 2013 Elsevier Ltd. All rights reserved.