Salidroside suppresses the metastasis of hepatocellular carcinoma cells by inhibiting the activation of the Notch1 signaling pathway
Salidroside suppresses the metastasis of hepatocellular carcinoma cells by inhibiting the activation of the Notch1 signaling pathway
复制标题
红景天苷通过抑制Notch1信号通路的激活抑制肝癌细胞的转移
DOI：
10.3892/mmr.2019.10115
复制
发表时间：
2019-06-01
影响因子：
3.4
通讯作者：
Xin, Yongning
中科院分区：
文献类型：
--
作者：
Lu, Linlin;Liu, Shousheng;Xin, Yongning
红景天苷（SDS）是从红景天中分离得到的一种苯丙苷，在临床医学中表现出多种药理特性，在中药中常用。本研究在体外研究了SDS对肿瘤侵袭和迁移的抑制作用，以及高转移性肝细胞癌（HCC）细胞（MHCC97H）中转移相关基因的表达。还探讨了 SDS 对肿瘤转移的潜在机制。研究发现 SDS 可显着减少伤口闭合面积并抑制细胞迁移。此外，SDS 显着抑制这些细胞侵入基质胶包被的膜。 SDS显着下调Notch1、Snail、COX-2、MMP-2、MMP-9基因的表达，并以剂量依赖性方式上调E-cadherin的表达。此外，SDS 抑制 Notch 信号靶基因 Hey1、Hes1 和 Hes5 的表达。总体而言，本研究结果表明SDS通过调节Notch1信号通路的活性来抑制HCC细胞转移。
Salidroside (SDS) is a phenylpropanoid glycoside isolated from Rhodiola rosea L. It exhibits multiple pharmacological properties in clinical medicine and has been commonly used in traditional Chinese medicine. The present study investigated the inhibitory effects of SDS on tumor invasion and migration, and the expression of metastasis-related genes in highly metastatic hepatocellular carcinoma (HCC) cells (MHCC97H) in vitro. The underlying mechanisms of SDS on the tumor metastasis were also explored. SDS was found to significantly reduce wound closure areas and inhibit cell migration. In addition, SDS markedly inhibited the invasion of these cells into Matrigel-coated membranes. SDS markedly downregulated the expression of Notch1, Snail, COX-2, MMP-2, MMP-9 genes and upregulated the expression of E-cadherin in a dose-dependent manner. Furthermore, SDS inhibited the expression of the Notch signaling target genes, Hey1, Hes1 and Hes5. On the whole, the findings of this study suggest that SDS inhibits HCC cell metastasis by modulating the activity of the Notch1 signaling pathway.