Enzyme-assisted synthesis of the glucuronide conjugate of psilocin, a hall ucinogenic component of magic mushroom
Enzyme-assisted synthesis of the glucuronide conjugate of psilocin, a hall ucinogenic component of magic mushroom
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酶辅助合成裸盖菇素（神奇蘑菇的霍尔致癌成分）的葡萄糖醛酸结合物
DOI：
10.1002/dta.244
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
T.Shoda
中科院分区：
文献类型：
--
作者：
Nurrochmad;et al.;T.Shoda
描述了裸盖菇素葡糖苷酸（PCG）的酶辅助合成，PCG是一种在神奇蘑菇（MM）使用者尿液中排泄的代谢物。在存在Aroclor 1254预处理的大鼠肝微粒体的情况下，将裸盖菇素和辅因子UDPGA孵育20 h。通过HPLC纯化得到PCG，产率为19%（3.6mg）。化合物结构经MS和NMR表征。通过该方法产生的PCG的毫克量将允许直接鉴定和定量MM使用者尿液中的PCG。版权所有© 2011约翰威利父子有限公司.
An enzyme‐assisted synthesis of psilocin glucuronide (PCG), a metabolite excreted in the urine of magic mushroom (MM) users, is described. In the presence of Aroclor 1254 pretreated rat liver microsomes, psilocin and the cofactor UDPGA were incubated for 20 h. Purification by HPLC gave PCG in 19% yield (3.6 mg). The compound structure was characterized by MS and NMR. The milligram amounts of PCG produced by this method will allow the direct identification and quantification of PCG in the urine of MM users. Copyright © 2011 John Wiley & Sons, Ltd.