Rat calmodulin cDNA.
Rat calmodulin cDNA.
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大鼠钙调蛋白 cDNA。
DOI：
10.1089/dna.1987.6.267
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Brosius,J
中科院分区：
文献类型：
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作者：
Sherbany,AA;Parent,AS;Brosius,J
我们报道了从大鼠脑cDNA库中分离到一个编码钙调蛋白的克隆；也分离了基因组克隆，并对其进行了部分鉴定。该氨基酸序列与已发表的哺乳动物蛋白质序列具有100%的同源性。在大鼠中，我们通过RNA印迹分析鉴定了两个主要的RNA物种，分别长约2.2kb和0.75kb。此外，与肝脏或肾脏相比，这种信使核糖核酸在脑组织中高度丰富。在其中一个mRNA物种的3‘端观察到了推测的PolyA加成信号的一个变体(GATAAA)。
We report the isolation of a clone encoding calmodulin from a rat brain cDNA library; genomic clones were also isolated and partially characterized. The derived amino acid sequence has 100% homology to the published mammalian protein sequences. In rat, we identify two major RNA species of approximately 2.2 and 0.75 kb in length by RNA blot analysis. Furthermore, the mRNA is highly enriched in brain tissue, compared to liver or kidney. A variant (GATAAA) of the putative poly(A) addition signal is observed at the 3′ end of one of the mRNA species.