Increased expression of the rice C-type cyclin-dependent protein kinase gene, Orysa;CDKC;1, in response to salt stress
Increased expression of the rice C-type cyclin-dependent protein kinase gene, Orysa;CDKC;1, in response to salt stress
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DOI：
10.1016/j.plaphy.2007.10.013
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发表时间：
2008-01-01
影响因子：
6.5
通讯作者：
Huang, Hao-Jen
中科院分区：
文献类型：
--
作者：
Huang, Ya-Wen;Tsay, Wen-Su;Huang, Hao-Jen
细胞周期蛋白依赖性蛋白激酶 (CDK) 形成保守的真核丝氨酸/苏氨酸蛋白激酶超家族，其活性需要与调节性细胞周期蛋白结合。 CDK 由多个基因家族组成，参与细胞生物学的不同方面，例如基因转录、细胞增殖和分化。在本研究中，我们介绍了水稻C型CDK基因Orysa;CDKC;1的特征。该基因在植物的所有器官中表达，并且在成熟的叶子和圆锥花序中检测到相对大量的转录本。这表明在叶子和穗发育的后期阶段具有假定的作用。使用酵母双杂交分析，我们确定了 Orysa;CYCT 与 Orysa;CDKC;1 的相互作用。将新的 Orysa;CDKC;1 响应水稻细胞各种因子的表达谱与先前描述的 Orysa;CDKA;1 和 Orysa;CDKB2;1 的表达谱进行比较。 Orysa;CDKC;1 转录物由盐胁迫和 ABA 处理诱导，但不受甘露醇影响。此外，与 Orysa;CDKC;1 免疫复合物相关的 MBP 激酶活性在盐胁迫下被诱导。我们认为 Orysa;CDKC;1 可能参与发育程序以及盐和 ABA 信号通路。 (c) 2007 年 Elsevier Masson SAS。版权所有。
Cyclin-dependent protein kinases (CDKs) form a conserved superfamily of eukaryotic serine/threonine protein kinases which require binding to a regulatory cyclin for activity. CDKs are organized in several gene families and are involved in different aspects of cell biology, such as gene transcription, cell proliferation and differentiation. In this study, we present the characteristics of the rice C-type CDK gene, Orysa;CDKC;1. The gene was expressed in all organs of the plant and relatively high amounts of its transcript were detected in mature leaves and panicles. This indicated a putative role during later stages of leaf and panicle development. Using yeast two-hybrid analysis, we identified Orysa;CYCT as an interactor with Orysa;CDKC;1. The expression profiles of the new Orysa;CDKC;1 in response to various factors from rice cells were compared with those of previously described Orysa;CDKA;1, and Orysa;CDKB2;1. The Orysa;CDKC;l transcript was induced by salt stress and ABA treatments, but was unaffected by mannitol. In addition, the MBP kinase activity associated with Orysa;CDKC;1 immunocomplexes was induced under salt stress. We propose that Orysa;CDKC;1 may be involved in developmental programs, as well as in the salt- and ABA-signaling pathway. (c) 2007 Elsevier Masson SAS. All rights reserved.