Developmental expression of class III and IV POU domain genes in the zebrafish.
Developmental expression of class III and IV POU domain genes in the zebrafish.
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斑马鱼中 III 类和 IV 类 POU 结构域基因的发育表达。
DOI：
10.1006/bbrc.1996.0274
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Stuart,GW
中科院分区：
文献类型：
--
作者：
Sampath,K;Stuart,GW
在基于PCR的克隆策略中使用冗余引物，在斑马鱼中克隆到了代表两个不同的III类基因(Brain-1.1，Brain-1.2)和一个IV类基因(Brain-3.1)的POU结构域编码序列。利用所构建的克隆对斑马鱼原肠胚基因文库进行筛选，获得了第三类POU基因(Brain-1.0)。用基因特异性引物进行的RT-PCR分析表明，这四个POU基因在发育中的胚胎和成鱼中表现出不同的表达模式。与它们的哺乳动物同源物一样，III类基因在发育早期和成人大脑中表达，而IV类基因在早期发育期间表达很低，但在成人眼睛中高表达。由于其中两个POU PCR克隆是从基因组DNA中获得的，鱼的基因可能也与它们的哺乳动物同源物相似，基本上没有内含子。
Using redundant primers in a PCR based cloning strategy, POU-domain encoding sequences representing two different class III genes (brain-1.1, brain-1.2) and one class IV gene (brain-3.1) were identified in zebrafish. Using the PCR clones to screen a zebrafish gastrula cDNA library, a third class III POU gene was identified (brain-1.0). RT–PCR assays performed with gene-specific primers indicated that the four POU genes exhibited distinct patterns of expression in developing embryos and adult fish. Like their mammalian homologs, the class III genes were expressed early in development and in the adult brain, while the class IV gene was poorly expressed during early development but highly expressed in the adult eye. Since two of the POU PCR clones were obtained from genomic DNA, the fish genes may also be similar to their mammalian homologs in being largely devoid of introns.