Comparative transcriptomic analysis of Clostridium acetobutylicum biofilm and planktonic cells
Comparative transcriptomic analysis of Clostridium acetobutylicum biofilm and planktonic cells
复制标题
丙酮丁醇梭菌生物膜和浮游细胞的比较转录组分析
DOI：
10.1016/j.jbiotec.2015.11.017
复制
发表时间：
2016-01-20
影响因子：
4.1
通讯作者：
Ying, Hanjie
中科院分区：
文献类型：
--
作者：
Liu, Dong;Xu, Jiahui;Ying, Hanjie
基于生物膜的产溶剂梭菌固定化已被广泛用于克服生物丁醇生产中的传统瓶颈，如溶剂毒性和低生产率。然而，很少有人探索产溶剂梭菌生物膜的分子基础。在此，我们首次报道了基于 DNA 阵列的丙酮丁醇梭菌生物膜细胞研究，以阐明转录调节。结果显示，生物膜细胞内丙酮丁醇梭菌基因组基因中有16.2%存在差异表达，其中大部分基因上调。最显着的变化发生在生物膜细胞中的氨基酸生物合成，其中硫吸收和半胱氨酸生物合成上调最多，而组氨酸生物合成下调最多。结果表明，丙酮丁醇梭菌生物膜细胞可能通过上调铁和硫的吸收以及Fe-S簇生物合成基因以及糖酵解基因来增加代谢活动。此外，参与孢子形成、颗粒形成、细胞外聚合物降解、戊糖分解代谢和各种其他过程的基因也受到显着调节，表明生物膜生长模式使细胞呈现独特的表型。这项研究为丙酮丁醇梭菌生物膜细胞的转录调控提供了有价值的见解，对于理解和开发基于生物膜的过程非常有用。 (C) 2015 Elsevier B.V. 保留所有权利。
Biofilm-based immobilization of solventogenic Clostridia has been extensively exploited to overcome traditional bottlenecks in biobutanol production like solvent toxicity and low productivities. However, the molecular basis of solventogenic Clostridia biofilm is rarely explored. Here, for the first time, we report DNA array-based study of Clostridium acetobutylicum biofilm cells to elucidate the transcriptional modulation. Results showed that 16.2% of the C. acetobutylicum genome genes within the biofilm cells were differentially expressed, with most genes being up-regulated. The most dramatic changes occurred with amino acid biosynthesis, with sulfur uptake and cysteine biosynthesis being the most up-regulated and histidine biosynthesis being the most down-regulated in the biofilm cells. It was demonstrated that C. acetobutylicum biofilm cells increased metabolic activities probably by up-regulating iron and sulfur uptake and Fe-S cluster biosynthesis genes as well as glycolysis genes. Furthermore, genes involved in sporulation, granulose formation, extracellular polymer degradation, pentose catabolisms, and various other processes were also notably regulated, indicating that the biofilm mode of growth rendered the cells a distinct phenotype. This study provides valuable insights into the transcriptional regulation in C. acetobutylicum biofilm cells and should be highly useful for understanding and developing the biofilm-based processes. (C) 2015 Elsevier B.V. All rights reserved.