Fiber kinematics of small intestinal submucosa under biaxial and uniaxial stretch
Fiber kinematics of small intestinal submucosa under biaxial and uniaxial stretch
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DOI：
10.1115/1.2354200
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发表时间：
2006-12-01
影响因子：
1.7
通讯作者：
Chancellor, Michael B.
中科院分区：
文献类型：
--
作者：
Gilbert, Thomas W.;Sacks, Michael S.;Chancellor, Michael B.
提高我们对生物来源的胶原支架的设计要求的理解对于它们在组织重建中的有效使用是必要的。在本研究中，利用小角光散射法(SALS)定量研究了在规定的单轴或双轴应变路径下，小肠粘膜下层(SIS)的胶原纤维运动学。使用了基于Billiar和Sack的改进的双向拉伸装置(J.Biomech，30，pp.753-7,1997)，由于SIS的自然半透明，使得对纤维运动学的实时分析成为可能。结果表明，无论加载路径如何，加载10%等轴应变的试件中胶原纤维的角分布与初始卸载时无显著差异(p=0.31)。10%的条状双向拉伸和大于5%的单轴拉伸导致胶原纤维沿同一方向的排列增加，而10%的条状双向拉伸导致胶原纤维沿交叉优先纤维方向的分布变宽。虽然仿射变形模型准确地预测了双轴应变状态的实验结果，但不能准确地预测单轴拉伸路径。为了全面预测SIS中大单轴应变下的纤维运动学，需要建立非仿射结构模型。
Improving our understanding of the design requirements of biologically derived collagenous scaffolds is necessary for their effective use in tissue reconstruction. In the present study, the collagen fiber kinematics of small intestinal submucosa (SIS) was quantified using small angle light scattering (SALS) while the specimen was subjected to prescribed uniaxial or biaxial strain paths. A modified biaxial stretching device based on Billiar and Sacks (J. Biomech., 30, pp. 753-7, 1997) was used, with a real-time analysis of the fiber kinematics made possible due to the natural translucency of SIS. Results indicated that the angular distribution of collagen fibers in specimens subjected to 10% equibiaxial strain was not significantly different from the initial unloaded condition, regardless of the loading path (p = 0.31). Both 10% strip biaxial stretch and uniaxial stretches of greater than 5% in the preferred fiber direction led to an increase in the collagen fiber alignment along the same direction, while 10% strip biaxial stretch in the cross preferred fiber direction led to a broadening of the distribution. While an affine deformation model accurately predicted the experimental findings for a biaxial strain state, uniaxial stretch paths were not accurately predicted. Nonaffine structural models will be necessary to fully predict the fiber kinematics under large uniaxial strains in SIS.