The use of magnetite-doped polymeric microspheres in calibrating cell tracking velocimetry
The use of magnetite-doped polymeric microspheres in calibrating cell tracking velocimetry
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DOI：
10.1016/s0165-022x(00)00085-3
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发表时间：
2000-07-10
影响因子：
--
通讯作者：
Chalmers, JJ
中科院分区：
文献类型：
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作者：
Moore, LR;Zborowski, M;Chalmers, JJ
连续磁分离是一个固有的动态过程，其中系统中没有质量积累，需要对可分离物质的先进知识以实现最佳仪器操作。通过在明确定义的场中确定细胞磁化，我们可以预测在我们的连续分离器的明确表征的场环境中的细胞轨迹行为。磁化强度是通过使用称为细胞跟踪测速法（CTV）的技术跟踪颗粒的迁移来确定的。CTV的验证需要根据外部标准进行校准。此外，需要这样一个标准，没有生物系统的变化和不稳定性，以参考该方法对日常变化或趋势。为此，我们发展了一种合成单分散、掺杂磁铁矿的高分子微球的方法。五套不同的磁铁矿含量的微球，每个直径约2.7 μ m，进行了研究。0.18 T/mm的平均梯度在CTV装置中诱导范围为0.45至420微米/s的磁性微球速度。速度使得能够计算微球磁化。磁力计测量允许在与CTV磁体的分析区域1.57 T的通量密度相当的通量密度下测定磁化强度。CTV和磁力计测量结果的比较显示出良好的一致性。(C)2000由Elsevier Science B. V.出版，保留所有权利。
Continuous magnetic separation, in which there is no accumulation of mass in the system, is an inherently dynamic process, requiring advanced knowledge of the separable species for optimal instrument operation. By determining cell magnetization in a well-defined field, we may predict the cell trajectory behavior in the well-characterized field environments of our continuous separators. Magnetization is determined by tracking the migration of particles with a technique known as cell tracking velocimetry (CTV). The validation of CTV requires calibration against an external standard. Furthermore, such a standard, devoid of the variations and instabilities of biological systems, is needed to reference the method against day-to-day shifts or trends. To this: end, a method of synthesizing monodisperse, magnetite-doped polymeric microspheres has been developed. Five sets of microspheres differing in their content of magnetite, and each of approximately 2.7 mu m diameter, are investigated. An average gradient of 0.18 T/mm induces magnetic microsphere velocities ranging from 0.45 to 420 microns/s in the CTV device. The velocities enable calculation of the microsphere magnetization. Magnetometer measurements permit the determination of magnetization at a flux density comparable to that of the CTV magnet's analysis region, 1.57 T. A comparison of the results of the CTV and magnetometer measurements shows good agreement. (C) 2000 Published by Elsevier Science B.V. All rights reserved.