Expression of semaphorin class 3 is higher in the proliferative phase on the human endometrium
Expression of semaphorin class 3 is higher in the proliferative phase on the human endometrium
复制标题
DOI：
10.1007/s00404-018-4719-3
复制
发表时间：
2018-05-01
影响因子：
2.6
通讯作者：
Germeyer, Ariane
中科院分区：
文献类型：
--
作者：
Ferreira, Gustavo Dias;Capp, Edison;Germeyer, Ariane
信号蛋白与血管生成和细胞增殖有关，具体取决于组织。本研究的目的是评估整个月经周期中人子宫内膜内 3 类信号蛋白 (SEMA3A-F) 的基因表达和信号蛋白 3A (SEMA3A) 的蛋白表达。在月经周期的增殖期和分泌期子宫内膜活检中，通过实时 PCR (qRT-PCR) 分析 SEMA3A-F 的基因表达，通过 ELISA 分析 SEMA3A 的蛋白表达。与增殖期子宫内膜相比，分泌期子宫内膜中 SEMA3A、SEMA3C、SEMA3D 和 SEMA3E 的表达显着降低（p < 0.05）。因此，分泌期的SEMA3A蛋白表达低于增殖期子宫内膜的蛋白表达（p<0.05）。SEMA3A、3C、3D和3E可能与子宫内膜细胞增殖有关，在周期的增殖期表达较多。这一发现可能会刺激 3 类信号蛋白作为治疗子宫内膜病理的可能靶点的研究。
The semaphorins are related to angiogenesis and cell proliferation depending on the tissue. The purpose of this study was to assess gene expression of class 3 semaphorin (SEMA3A-F) and protein expression of semaphorin 3A (SEMA3A) within human endometrium throughout the menstrual cycle.Gene expression of SEMA3A-F was analyzed by real-time PCR (qRT-PCR) and protein expression of SEMA3A was analyzed by ELISA in endometrial biopsies in the proliferative and secretory phase of the menstrual cycle.Gene expression of SEMA3A, SEMA3C, SEMA3D, and SEMA3E was statistically significant decreased in secretory compared to proliferative phase endometrium (p < 0.05). Accordingly, SEMA3A protein expression in the secretory phase was lower than protein expression in proliferative phase endometrium (p 0.05).SEMA3A, 3C, 3D, and 3E are possibly related to cell proliferation in the endometrium, being more expressed in the proliferative phase of the cycle. This finding may stimulate studies of class 3 semaphorins as a possible target for treatment of endometrial pathologies.