Regional dependency of bovine meniscus biomechanics on the internal structure and glycosaminoglycan content
Regional dependency of bovine meniscus biomechanics on the internal structure and glycosaminoglycan content
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DOI：
10.1016/j.jmbbm.2019.02.020
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发表时间：
2019-06-01
影响因子：
3.9
通讯作者：
Collins, Maurice N.
中科院分区：
文献类型：
--
作者：
Murphy, Caroline A.;Cunniffe, Grainne M.;Collins, Maurice N.
半月板在膝关节的机械功能中发挥着重要作用。它们受到很大的压缩力，因此，由于其无血管结构，半月板很容易受到不可修复的损伤。半月板切除术曾经是受损半月板的常见手术，但目前人们对涉及半月板再生以恢复功能的替代方法感兴趣。为了实现这些再生策略，最初建立天然半月板的结构/特性/功能关系至关重要。因此，本研究探讨半月板细胞外基质主要成分的影响；即糖胺聚糖（GAG）和胶原纤维取向对牛半月板机械性能的影响。 GAG 分布和机械特性根据半月板内的深度和区域差异进行映射。结果表明，与周边区域相比，半月板内部区域的 GAG 含量显着增加。胫骨和股骨层含有比中段更多的 GAG，并且胶原纤维排列因区域而异。总体而言，可以确定弯液面的粘弹性特性是由弯液面的固体部分和流体部分之间的相互依赖关系决定的，并且这种关系根据区域而变化。 GAG 分子的亲水性在维持固/液平衡方面发挥着重要作用，而胶原纤维方向限制了组织内的流体流动，结合这些过程可在压缩载荷下支撑半月板。
Menisci play a major role in the mechanical function of the knee. They are subjected to large compressive forces and as a result and due to its avascular structure, menisci are prone to irreparable damage. Meniscectomy was once a common procedure for damaged menisci, however alternative approaches involving meniscus regeneration to restore function are of current interest. In order to enable these regenerative strategies, it is of utmost importance to initially establish he structure/property/function relationships of native menisci. Therefore, this study explores the influence of major constituents of the meniscal extracellular matrix; namely the glycosaminoglycan (GAG), and the collagen fibre orientation on the mechanical properties of the bovine meniscus. GAG distribution and mechanical properties are mapped with respect to depth and regional variance within the meniscus. Results show that the inner zone of the meniscus has a significantly larger quantity of GAG compared to the peripheral zone. The tibial and femoral layers contain a higher quantity of GAG than the midsection and collagen fibre alignment differed depending on region. Overall, it was established that the viscoelastic properties of the meniscus are determined by the co-dependent relationship between the solid and fluid fractions of the meniscus and this varied depending on region. The hydrophilic nature of the GAG molecules play an important role in maintaining the solid/fluid balance while collagen fibre orientation restricts fluid flow within tissue, combined these processes act to support the meniscus under compressive loads.