Four differentially methylated gene pairs to predict the prognosis for early stage hepatocellular carcinoma patients
Four differentially methylated gene pairs to predict the prognosis for early stage hepatocellular carcinoma patients
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DOI：
10.1002/jcp.26256
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发表时间：
2018-09-01
影响因子：
5.6
通讯作者：
Lu, Xiao-Jie
中科院分区：
文献类型：
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作者：
Liu, Shaoguang;Miao, Changfeng;Lu, Xiao-Jie
肝细胞癌是第二大常见恶性肿瘤，预后较差。我们的目标是识别新的甲基化特征来预测早期肝细胞癌患者。用SAM方法筛选TCGA检索到的肝细胞癌患者和正常肝组织中差异表达的甲基化基因。考克斯发现了与患者生存高度相关的基因。通过前向搜索算法识别出能够识别复发肝癌患者的特征。此外，对签名中的甲基化基因进行了功能富集化分析。共筛选出5,392个差异表达的CpG位点，获得了4,294个差异表达基因。共有197个基因与肝细胞癌I期和II期患者的RFS相关。通过C指数前向搜索法获得了由21对组成的特征来预测预后情况。这些基因的功能通过功能富集化分析得到。综上所述，该标记由21对基因组成，可以预测肝癌患者I期和II期的预后情况，为肝癌患者术后的辅助治疗提供了参考标准。
Hepatocellular carcinoma (HCC) was the second most common malignant tumor with a poor prognostic condition. We aimed to identify novel methylation signatures to predict HCC patients at their early stages. Differentially expressed methylated genes between HCC patients and normal liver tissues retrieved from TCGA were screened out by SAM. Genes highly related to patients' survival were figured out by COX. The signatures that could identify relapse HCC patients were identified by the forwarding search algorithm. Besides, functional enrichment analysis was performed on the methylation genes in the signature. A total of 5,392 CPG sites that differentially methylated expressed were found out and 4,294 differentially expressed genes were obtained. A total of 197 genes among were associated with RFS of HCC patients at both stage I and stage II. Signature composed of 21 pairs was obtained to predict the prognostic situation by C-index forward search method. The function of these genes was figured out by functional enrichment analysis. To summary, the signature composed of 21 gene pairs that can predict the prognostic situation of HCC patients at both stage I and stage II, provided a reference standard for the adjuvant therapy of HCC patients after surgery.