Retinal Structure of Birds of Prey Revealed by Ultra-High Resolution Spectral-Domain Optical Coherence Tomography
Retinal Structure of Birds of Prey Revealed by Ultra-High Resolution Spectral-Domain Optical Coherence Tomography
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DOI：
10.1167/iovs.10-5633
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发表时间：
2010-11-01
影响因子：
4.4
通讯作者：
Jiao, Shuliang
中科院分区：
文献类型：
--
作者：
Ruggeri, Marco;Major, James C., Jr.;Jiao, Shuliang
目的.揭示猛禽视网膜结构的三维信息。建立了一套用于猛禽视网膜活体成像的超高分辨率光谱域光学相干层析成像系统。系统的校准成像深度和轴向分辨率分别为3.1 mm和2.8 μ m（在组织中）。3-D分割进行计算的视网膜神经纤维层（RNFL）的地图。高分辨率OCT图像获得的视网膜的四种猛禽：两个昼夜鹰（Buteo platypterus和Buteo brachyurus）和两个夜间猫头鹰（Bubo virginianus和Strix varia）。这些图像显示了详细的视网膜解剖结构，包括视网膜层和深凹和浅凹的结构。计算的厚度图显示了RNFL分布。一只鸟的视网膜外伤也成功成像。超高分辨率SD-OCT为猛禽的视网膜结构提供了前所未有的高质量2-D和3-D活体可视化。SD-OCT是猛禽视觉研究的强大成像工具。（Invest Ophthalmol维斯科学。2010; 51：5789-5795）DOI：10.1167/iovs.10-5633
PURPOSE. To reveal three-dimensional (3-D) information about the retinal structures of birds of prey in vivo.METHODS. An ultra-high resolution spectral-domain optical coherence tomography (SD-OCT) system was built for in vivo imaging of retinas of birds of prey. The calibrated imaging depth and axial resolution of the system were 3.1 mm and 2.8 mu m (in tissue), respectively. 3-D segmentation was performed for calculation of the retinal nerve fiber layer (RNFL) map.RESULTS. High-resolution OCT images were obtained of the retinas of four species of birds of prey: two diurnal hawks (Buteo platypterus and Buteo brachyurus) and two nocturnal owls (Bubo virginianus and Strix varia). These images showed the detailed retinal anatomy, including the retinal layers and the structure of the deep and shallow foveae. The calculated thickness map showed the RNFL distribution. Traumatic injury to one bird's retina was also successfully imaged.CONCLUSIONS. Ultra-high resolution SD-OCT provides unprecedented high-quality 2-D and 3-D in vivo visualization of the retinal structures of birds of prey. SD-OCT is a powerful imaging tool for vision research in birds of prey. (Invest Ophthalmol Vis Sci. 2010; 51:5789-5795) DOI:10.1167/iovs.10-5633