A novel turn-on fluorescent probe for Hg2+ detection based on rhodamine B spirolactam derivative
A novel turn-on fluorescent probe for Hg2+ detection based on rhodamine B spirolactam derivative
复制标题
基于罗丹明 B 螺内酰胺衍生物的新型 Hg2 检测开启荧光探针
DOI：
10.1080/03067319.2019.1625339
复制
发表时间：
2019-06-09
影响因子：
2.6
通讯作者：
Yu, Yanhua
中科院分区：
文献类型：
--
作者：
Dan, Wen;Deng, Xuankai;Yu, Yanhua
报道了一种基于罗丹明B螺内酰胺的新型荧光探针1。在所测试的金属离子中，探针1在甲醇和0.02 M HEPES缓冲液（V/V = 9：1，pH = 7.2）的混合溶液中对Hg ~（2+）表现出较高的选择性。在450 ~ 700 nm范围内没有观察到探针1的吸收和发射带。当Hg 2+加入探针1中时，在520 nm激发下，探针1在553 nm处出现一个新的吸收带，在577 nm附近出现一个新的荧光发射带。在1.6 ~ 32 μ M范围内，Hg ~（2+）浓度与577 nm处的荧光强度呈良好的线性关系（R ~（-2）= 0.9993）。通过H-1 NMR、MS、吸收光谱和荧光光谱等分析，证实了Hg 2+诱导螺内酰胺开环的机理。此外，探针1还能检测到真实的水样和滤纸上的Hg 2+，表明了其在环境科学中的应用。
In this work, a new turn-on fluorescent probe 1 for Hg2+ ions detection based on rhodamine B spirolactam was reported. Among tested metal ions, probe 1 shows high selectivity towards Hg2+ in the the mixture solution of methanol and 0.02 M HEPES buffer (V/V = 9:1, pH = 7.2). No absorption and emission band of probe 1 was observed in the range from 450 to 700 nm. While only addition of Hg2+ to probe 1 could lead to appearance of a new absorption band centered at 553 nm and a fluorescence emission band around 577 nm upon excitation at 520 nm. Moreover, it exhibits excellent linear relationship (R-2 = 0.9993) between fluorescence intensity at 577 nm and the concentration of Hg2+ from 1.6 to 32 mu M. The sensing mechanism was proven to be spirolactam ring open induced by Hg2+ through H-1 NMR, MS, absorption and fluorescence spectra. In addition, probe 1 could detect Hg2+ in real water samples and on filter paper, which demonstrates its application in environment science.