Effects of bacterial superantigens on behavior of mice in the elevated plus maze and light-dark box.
Effects of bacterial superantigens on behavior of mice in the elevated plus maze and light-dark box.
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细菌超抗原对高架十字迷宫和明暗盒中小鼠行为的影响。
DOI：
10.1016/s0889-1591(03)00087-4
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Kusnecov,AlexanderW
中科院分区：
文献类型：
--
作者：
Rossi-George,Alba;LeBlanc,Fred;Kaneta,Takehiro;Urbach,Daniella;Kusnecov,AlexanderW
细菌超抗原，如葡萄球菌肠毒素A和B(SEA/SEB)刺激T细胞在血液中产生高水平的细胞因子。以前已经证明，这些毒素能够刺激神经内分泌活性增加，并增强对新的味觉和非味觉刺激的行为反应。因此，有人认为这些超抗原可能促进焦虑样行为。在目前的一系列实验中，BALB/cByJ和C57BL/6J雄性小鼠分别被SEB(50μg)或SEA(5或10μg)挑战，并在高架+迷宫中测试行为。结果表明，SEA或SEB治疗没有增加焦虑样行为，张开双臂的探索得到加强。在另一项焦虑测试中，BALB/cByJ小鼠在测试前90分钟进行的明暗盒子SEB挑战，没有改变退出潜伏期、活动或在黑暗中度过的时间。然而，在第二个实验中，研究发现，如果首先测试动物的消费，然后在明暗盒子中测试，SEB挑战的动物表现出更长的退出潜伏期和更少的探索。这些研究表明，在啮齿动物焦虑样行为的标准测试中，用SEA或SEB挑战后诱导焦虑样过程的证据并不明显。然而，免疫刺激诱导的神经生物学事件可能与对心理和/或味觉刺激的反应协同作用，从而导致焦虑样行为增加，这可能是标准行为测试(如明暗盒子或EPM)所不能掩盖的。
Bacterial superantigens, such as staphylococcal enteroxins A and B (SEA/SEB) stimulate T cells to produce high levels of cytokines in blood. Previously it had been shown that these toxins were capable of stimulating increased neuroendocrine activity and enhanced behavioral reactivity to novel gustatory and non-gustatory stimuli. Therefore, it was suggested that these superantigens may promote anxiety-like behavior. In the current set of experiments, BALB/cByJ and C57BL/6J male mice were challenged with either SEB (50μg) or SEA (5 or 10μg) and tested for behavior in the elevated plus maze (EPM). Results suggested an absence of increased anxiety-like behavior, with exploration of the open arms being enhanced by SEA or SEB treatment. In another test of anxiety, the light–dark box, SEB challenge of BALB/cByJ mice 90min prior to testing, did not alter exit latency, activity nor time spent in the dark. However, in a second experiment, it was found that if animals were first tested for consumption, followed by testing in the light–dark box, SEB challenged animals displayed increased exit latency and reduced exploration. These studies suggest that in standard tests of rodent anxiety-like behavior, evidence for the induction of anxiety-like processes subsequent to challenge with SEA or SEB is not patently discernable. However, neurobiological events induced by immunological challenge might synergize with reactivity to psychogenic and/or gustatory stimuli, thereby resulting in increased anxiety-like behavior that could be unmasked by standard behavioral tests such as the light–dark box or EPM.