SWAP-70 is required for oncogenic transformation by v-Src in mouse embryo fibroblasts
SWAP-70 is required for oncogenic transformation by v-Src in mouse embryo fibroblasts
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DOI：
10.1016/j.bbrc.2007.03.011
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发表时间：
2007-05-04
影响因子：
3.1
通讯作者：
Ihara, Sayoko
中科院分区：
文献类型：
--
作者：
Fukui, Yasuhisa;Tanaka, Takayuki;Ihara, Sayoko
SWAP-70是三磷酸磷脂酰肌醇（PtdIns（3，4，5）P-3）结合蛋白，其在F-肌动蛋白重排中起作用。通过使用SWAP-70缺陷的小鼠胚胎成纤维细胞（MEFs）来检查SWAP-70在由v-Src引起的MEFs的致癌转化中的作用。v-Src形态学转化缺乏SWAP-70的MEFs，但转化细胞在培养物中的生长比补充有外源SWAP-70的细胞慢。缺乏SWAP-70的v-Src转化的MEFs不能在软琼脂中生长，而表达SWAP-70的MEFs容易形成集落，这表明SWAP-70是v-Src转化的MEFs的锚定非依赖性生长所必需的。当移植到裸鼠中时，由v-Src转化的SWAP-70（-/-）MEFs形成的肿瘤小于由表达外源性SWAP-70的细胞形成的肿瘤。这些结果表明，SWAP-70可能是致癌转化所必需的，并有助于v-Src转化的MEFs中的细胞生长。(c)2007年爱思唯尔公司All rights reserved.
SWAP-70 is a phosphatidylinositol trisphosphate (PtdIns(3,4,5)P-3) binding protein, which acts in F-actin rearrangement. The role of SWAP-70 in oncogenic transformation of mouse embryo fibroblasts (MEFs) by v-Src was examined by use of MEFs defective in SWAP-70. v-Src morphologically transformed MEFs lacking SWAP-70, but growth of the transformed cells in culture was slower than that of cells supplemented with exogenous SWAP-70. The v-Src-transformed MEFs deficient in SWAP-70 were unable to grow in soft agar while those expressing SWAP70 readily formed colonies, suggesting that SWAP-70 is required for anchorage independent growth of v-Src transformed MEFs. When transplanted in nude mice, tumors formed by the v-Src transformed SWAP-70(-/-) MEFs were smaller than those formed by cells expressing exogenous SWAP-70. These results suggest that SWAP-70 may be required for oncogenic transformation and contributes to cell growth in MEFs transformed by v-Src. (c) 2007 Elsevier Inc. All rights reserved.