The inhibitory effect of locally injected dexmedetomidine on carrageenan-induced nociception in rats.
The inhibitory effect of locally injected dexmedetomidine on carrageenan-induced nociception in rats.
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局部注射右美托咪定对角叉菜胶引起的大鼠伤害感受的抑制作用。
DOI：
10.1016/j.ejphar.2015.06.054
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发表时间：
2015
影响因子：
5
通讯作者：
Miyawaki T.
中科院分区：
文献类型：
--
作者：
Honda Y;Higuchi H;Matsuoka Y;Yabuki-Kawase A;Ishii-Maruhama M;Tomoyasu Y;Maeda S;Morimatsu H;Miyawaki T.
最近的研究表明，右美托咪啶的施用缓解了存在神经性疼痛的痛觉过敏。这些发现导致了右美托咪定局部给药可用于缓解急性炎性伤害感受（如术后疼痛）的假设。因此，我们评估了局部注射右美托咪定对急性炎症性伤害感受的抑制作用。通过向大鼠后爪足底注射1%角叉菜胶诱导急性炎性伤害感受，并将右美托咪定与角叉菜胶联合注射。测量基于von Frey细丝刺激的缩爪阈值，直至注射后12小时。我们比较了角叉菜胶和角叉菜胶与右旋美托咪啶之间的时间曲线下面积（AUC）。为了阐明右美托咪定的作用是通过α2-肾上腺素受体，我们评价了育亨宾（α2-肾上腺素受体的选择性拮抗剂）对右美托咪定抗伤害性感受的影响。结果，与仅注射角叉菜胶相比，足底注射角叉菜胶和超过10 μM右美托咪啶显著增加AUC。通过将育亨宾添加到角叉菜胶和右美托咪定中来逆转右美托咪定的这种作用。这些结果表明，局部注射右美托咪定可通过α2-肾上腺素受体有效对抗角叉菜胶诱导的炎性伤害感受。结果表明，局部注射右美托咪定可有效减轻局部急性炎症伤害性感受。
Recent studies showed that the administration of dexmedetomidine relieved hyperalgesia in the presence of neuropathic pain. These findings have led to the hypothesis that the local administration of dexmedetomidine is useful for relieving acute inflammatory nociception, such as postoperative pain. Thus, we evaluated the inhibitory effect of locally injected dexmedetomidine on acute inflammatory nociception. Acute inflammatory nociception was induced by an intraplantar injection of 1% carrageenan into the hindpaws of rats, and dexmedetomidine was also injected combined with carrageenan. The paw withdrawal threshold based on von Frey filament stimulation was measured until 12 h after injection. We compared the area under the time-curve (AUC) between carrageenan and carrageenan with dexmedetomidine. To clarify that the action of dexmedetomidine was via α2-adrenoceptors, we evaluated the effect of yohimbine, a selective antagonist of α2-adrenoceptors, on the anti-nociception of dexmedetomidine. As the results, the intraplantar injection of carrageenan with over 10 μM dexmedetomidine significantly increased AUC, compared to that with only carrageenan injection. This effect of dexmedetomidine was reversed by the addition of yohimbine to carrageenan and dexmedetomidine. These results demonstrated that the locally injected dexmedetomidine was effective against carrageenan-induced inflammatory nociception via α2-adrenoceptors. The findings suggest that the local injection of dexmedetomidine is useful for relieving local acute inflammatory nociception.