CYTOGENETICS OF SOMATIC-CELL HYBRIDS .2. MODAL KARYOTYPES AND MOSAICISM IN MOUSE MUTANT-CELL LINES - LM(TK-), A9, AND RAG
CYTOGENETICS OF SOMATIC-CELL HYBRIDS .2. MODAL KARYOTYPES AND MOSAICISM IN MOUSE MUTANT-CELL LINES - LM(TK-), A9, AND RAG
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DOI：
10.1016/0165-4608(87)90086-0
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发表时间：
1987-01-01
影响因子：
--
通讯作者：
CHEN, TR
中科院分区：
文献类型：
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作者：
CHEN, TR
小鼠突变细胞系LM（tk-）、A9和Rag经常被用作体细胞杂交的供体细胞系，本文描述了这些细胞系的模态核型，并与其他实验室先前报道的核型进行了比较。结果总结如下。每个品系中有许多细胞（18% ~ 38%）的染色体组成与模态核型相同。在某些细胞中，模态核型的染色体差异主要涉及一条或几条染色体。这些差异主要是由记录良好的机制引起的结构变化或简单的数值变化引起的，包括细胞破裂引起的伪影。同一细胞系内共存亚群的核型可以清楚地确定。对异倍体细胞群体核特性的评估可能会有很大的不同，这取决于它是对一个亚群体还是作为一个单位的总体群体进行评估。这些细胞系的模态核型包含大多数的标记染色体，并且与其他实验室先前报道的染色体组成相似。这些细胞系表现出核型的稳定性；因此，我们相信与体细胞杂交克隆生长相关的染色体变化可以毫无歧义地确定。
Modal karyotypes of mouse mutant cell lines, LM(tk-), A9, and Rag, which are frequently used as a donor line for somatic cell hybridization, are described and compared with karyotpes previously reported by other laboratories. Results are summarized as follows. Many cells (18%-38%) in each line had the same chromosome composition as the modal karyotype. Chromosome differences from the modal karytotype in some cells mainly involved one or a few chromosomes only. These differences are mostly caused by structural changes from well documented mechanisms or simple numerical changes including artifacts arising from the rupture of cells. Karyotypes of coexisting subpopulations within the same cell line can be clearly identified. Assessment of karyologic properties of a heteroploid cell population may vary considerably depending on whether it is estimated on a subpopulation or the total population as a unit. Modal karyotypes of these cell lines contained most marker chromosomes of, and a similar chromosome composition to those previously reported by other laboratories. These cell lines exhibit karyotypic constancy; thus, we believe that chromosome changes associated with clonal growth of somatic cell hybrids can be identified without ambiguity.