MOLECULAR EPIDEMIOLOGY OF RABIES VIRUS IN FRANCE - COMPARISON WITH VACCINE STRAINS
MOLECULAR EPIDEMIOLOGY OF RABIES VIRUS IN FRANCE - COMPARISON WITH VACCINE STRAINS
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DOI：
10.1099/0022-1317-73-5-1149
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发表时间：
1992-05-01
影响因子：
3.8
通讯作者：
TORDO, N
中科院分区：
文献类型：
--
作者：
SACRAMENTO, D;BADRANE, H;TORDO, N
通过聚合酶链反应扩增基因直接测序，对目前在法国流行的狂犬病病毒进行了分子流行病学研究。狂犬病病毒假基因PSI被选为最具差异的基因组区域，因此是测量病毒进化的最佳“时钟”。12个野生狂犬病病毒分离株之间的序列比较表明，无论宿主和病毒分离的地方，都具有很强的保守性。这适用于一个独特的野生水库，狐狸。另一方面，遗传和地理标准之间的良好相关性表明野生病毒的缓慢进化与动物流行病的时空进展平行。与其内在的同质性（约2%的分歧）相反，野生分离株序列显示出与疫苗种子株序列的显著分歧（约14.7%）。这一发现需要在世界范围内进行分子流行病学研究，特别是在已报告疫苗接种失败的国家。
A molecular epidemiological study of the rabies virus currently prevalent in France was carried out by directly sequencing polymerase chain reaction-amplified genes. The rabies virus pseudogene-PSI was chosen as the most divergent genomic area, and as such the best 'clock' for measuring virus evolution. Sequence comparisons between 12 wild rabies virus isolates indicated strong conservation whatever the host and wherever the virus had been isolated. This holds true for a unique wild reservoir, the fox. On the other hand, a good correlation between genetic and geographical criteria indicates a slow evolution of the wild virus in parallel with the spatio-temporal progression of the epizootic. In contrast to their intrinsic homogeneity (about 2% divergence), the wild isolate sequences showed a marked divergence from those of vaccine seed strains (about 14.7%). This finding invites world-wide molecular epidemiological studies, particularly in countries in which vaccination failures have been reported.