Gelatin Scaffolds with Controlled Pore Structure and Mechanical Property for Cartilage Tissue Engineering
Gelatin Scaffolds with Controlled Pore Structure and Mechanical Property for Cartilage Tissue Engineering
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DOI：
10.1089/ten.tec.2015.0281
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发表时间：
2016-03-01
影响因子：
3
通讯作者：
Chen, Guoping
中科院分区：
文献类型：
--
作者：
Chen, Shangwu;Zhang, Qin;Chen, Guoping
使用多孔支架和软骨细胞体外工程软骨组织为软骨修复提供了一种有前途的方法。然而，体外工程软骨的不均匀细胞分布和异质组织形成以及较弱的机械性能限制了其临床应用。在本研究中，利用冰颗粒和冷冻干燥制备了具有均匀开孔的明胶多孔支架。该支架用于培养牛关节软骨细胞，以在体外改造软骨组织。通过使用不同的冰粒与明胶溶液的比例以及不同的明胶浓度，可以很好地控制明胶支架的孔结构和力学性能。由 70% 冰颗粒制备的明胶支架能够使牛关节软骨细胞均匀接种在整个支架上，并形成均匀的软骨细胞外基质。软支架经历细胞收缩，而硬支架抵抗细胞收缩，并且具有显着更高的细胞增殖和硫酸化糖胺聚糖的合成。与没有冰颗粒制备的明胶支架相比，用冰颗粒制备的明胶支架有利于形成均质的软骨组织，且压缩模量明显更高。明胶支架具有高度开孔结构和良好的机械性能，可用于改善体外组织工程软骨。
Engineering of cartilage tissue in vitro using porous scaffolds and chondrocytes provides a promising approach for cartilage repair. However, nonuniform cell distribution and heterogeneous tissue formation together with weak mechanical property of in vitro engineered cartilage limit their clinical application. In this study, gelatin porous scaffolds with homogeneous and open pores were prepared using ice particulates and freeze-drying. The scaffolds were used to culture bovine articular chondrocytes to engineer cartilage tissue in vitro. The pore structure and mechanical property of gelatin scaffolds could be well controlled by using different ratios of ice particulates to gelatin solution and different concentrations of gelatin. Gelatin scaffolds prepared from 70% ice particulates enabled homogeneous seeding of bovine articular chondrocytes throughout the scaffolds and formation of homogeneous cartilage extracellular matrix. While soft scaffolds underwent cellular contraction, stiff scaffolds resisted cellular contraction and had significantly higher cell proliferation and synthesis of sulfated glycosaminoglycan. Compared with the gelatin scaffolds prepared without ice particulates, the gelatin scaffolds prepared with ice particulates facilitated formation of homogeneous cartilage tissue with significantly higher compressive modulus. The gelatin scaffolds with highly open pore structure and good mechanical property can be used to improve in vitro tissue-engineered cartilage.