Precise localization of aphidicolin-induced breakpoints on the short arm of human chromosome 3.
Precise localization of aphidicolin-induced breakpoints on the short arm of human chromosome 3.
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DOI：
10.1006/geno.1995.1057
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发表时间：
1995-05
期刊：
影响因子：
4.4
通讯作者：
W. Paradee;C. Mullins;Z. He;T. Glover;C. Wilke;B. Opalka;J. Schutte;D. Smith
中科院分区：
文献类型：
--
作者：
W. Paradee;C. Mullins;Z. He;T. Glover;C. Wilke;B. Opalka;J. Schutte;D. Smith
3p14.2处的共同脆性位点（FRA 3B）被认为是人类基因组中最活跃的脆性位点。该基因座可能使3号染色体易发生特定缺失和易位，这些缺失和易位与几种恶性肿瘤（包括遗传性肾细胞癌）有关。我们先前已经描述了诱导断裂FRA 3B周围使用aphidicolin在体细胞杂交，其唯一的人类组成部分是一个单一的完整的3号染色体。这项工作导致分离出在3 p13-p21.1区域具有断裂点的杂交体，其各自断裂点远端的所有序列均丢失。在这份报告中，我们描述了进一步表征的断点在许多这些细胞系使用新的可用的分子标记。我们还报告了鉴定跨越断点存在于许多这些杂交种的YAC克隆。
The common fragile site at 3p14.2 (FRA3B) has been described as the most active fragile site in the human genome. This locus may predispose chromosome 3 to specific losses due to deletions and translocations that have been associated with several malignancies, including hereditary renal cell carcinoma. We have previously described induction of breakage around FRA3B using aphidicolin in a somatic cell hybrid whose only human component was a single intact chromosome 3. That work led to the isolation of hybrids with breakpoints in the 3p13-p21.1 region with loss of all sequences distal to their respective breakpoints. In this report we describe the further characterization of the breakpoints in many of these cell lines using newly available molecular markers. We also report the identification of YAC clones that span the breakpoints present in many of these hybrids.