Functional Divergence of the Arabidopsis Florigen-Interacting bZIP Transcription Factors FD and FDP
Functional Divergence of the Arabidopsis Florigen-Interacting bZIP Transcription Factors FD and FDP
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DOI：
10.1016/j.celrep.2020.107717
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发表时间：
2020-06
期刊：
影响因子：
8.8
通讯作者：
Maida Romera-Branchat;E. Severing;Chloé Pocard;Hyonhwa Ohr;C. Vincent;G. Née;Rafael Martínez-Gallegos-Rafael-Martínez-Gal
中科院分区：
文献类型：
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作者：
Maida Romera-Branchat;E. Severing;Chloé Pocard;Hyonhwa Ohr;C. Vincent;G. Née;Rafael Martínez-Gallegos-Rafael-Martínez-Gal
许多植物的开花依赖于碱性亮氨酸拉链（bZIP）转录因子和系统转运的成花素蛋白之间的相互作用。成员的genusopsis包含两个这些bZIPs，FD和FDP，我们表明有很大程度上互补的表达模式，在茎尖开花前和开花期间。CRISPR-Cas9诱导的FDP无效突变体比野生型稍早开花，而fDP突变体开花较晚。相同的G-box序列在FD和FDP结合位点富集，但只有FD与开花相关基因结合，并且仅影响其转录。然而，这两种蛋白质都与参与对植物激素脱落酸（阿坝）反应的基因结合，ABA控制发育和应激反应。这些基因中的许多基因在fdp和fdp突变体幼苗中差异表达，这也表明阿坝敏感性降低。因此，成花素相互作用的bZIPs在开花中具有不同的功能，取决于它们的表达模式，并且在发育的早期阶段，在植物激素信号传导中发挥共同的作用。
Flowering of many plant species depends on interactions between basic leucine zipper (bZIP) transcription factors and systemically transported florigen proteins. Members of the genusArabidopsiscontain two of these bZIPs, FD and FDP, which we show have largely complementary expression patterns in shoot apices before and during flowering. CRISPR-Cas9-induced null mutants forFDPflower slightly earlier than wild-type, whereasfdmutants are late flowering. Identical G-box sequences are enriched at FD and FDP binding sites, but only FD binds to genes involved in flowering and onlyfdalters their transcription. However, both proteins bind to genes involved in responses to the phytohormone abscisic acid (ABA), which controls developmental and stress responses. Many of these genes are differentially expressed in bothfdandfdpmutant seedlings, which also show reduced ABA sensitivity. Thus, florigen-interacting bZIPs have distinct functions in flowering dependent on their expression patterns and, at earlier stages in development, play common roles in phytohormone signaling.