Cloning, renal distribution, and regulation of the rat Na+-HCO3- cotransporter
Cloning, renal distribution, and regulation of the rat Na+-HCO3- cotransporter
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DOI：
10.1152/ajprenal.1998.274.6.f1119
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发表时间：
1998-06-01
影响因子：
4.2
通讯作者：
Soleimani, M
中科院分区：
文献类型：
--
作者：
Burnham, CE;Flagella, M;Soleimani, M
我们最近报道了人肾Na+-HCO 3-共转运蛋白（NBC-1）的克隆和表达（C. E. Burnham，H. Amlal，Z. Wang，G. E. Shull和M.索莱马尼272：19111-19114，1997）。为了加快体内实验，我们现在报告大鼠肾脏NBC-1的cDNA序列。此外，我们描述了器官和肾单位段的分布和NBC-1 mRNA的调节下的三种模型的pH应激：氯耗竭性水肿（CDA），代谢性酸中毒，和碳酸氢盐负荷。大鼠NBC-1 cDNA编码1，035个氨基酸的开放阅读框，与人和蝾螈NBC-1的同源性分别为96%和87%。大鼠NBC-1 mRNA在肾脏和脑中以高水平表达，在结肠、胃和心脏中以较低水平表达。肝内未见。在肾脏中，NBC-1主要在近端小管中表达，在髓质中发现痕量。上升肢和乳头。HCO_3负荷降低了NBC-1 mRNA的表达。水平，这是不变的代谢性酸中毒或CDA。
We recently reported the cloning and expression of a human kidney Na+-HCO3- co-transporter (NBC-1) (C. E. Burnham, H. Amlal, Z. Wang, G. E. Shull, and M. Soleimani. J. Biol. Chem. 272: 19111-19114, 1997). To expedite in vivo experimentation, we now report the cDNA sequence of rat kidney NBC-1. In addition, we describe both the organ and nephron segment distributions and the regulation of NBC-1 mRNA under three models of pH stress: chloride-depletion alkalosis (CDA), metabolic acidosis, and bicarbonate loading. Rat NBC-1 cDNA encodes an open reading frame of 1,035 amino acids, with 96 and 87% identity to human and salamander NBC-1, respectively. Rat NBC-1 mRNA is expressed at high levels in kidney and brain, with lower levels in colon, stomach, and heart. None appears in liver. In the kidney, NBC-1 is expressed mainly in the proximal tubule, with traces found in medullary thick. ascending limb and papilla. HCO3- loading decreased NBC-1 mRNA. levels, which were unchanged either by metabolic acidosis or by CDA.