Nucleotide sequence and transcriptional analysis of the Streptomyces griseus gene (afsA) responsible for A-factor biosynthesis
Nucleotide sequence and transcriptional analysis of the Streptomyces griseus gene (afsA) responsible for A-factor biosynthesis
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负责 A 因子生物合成的灰色链霉菌基因 (afsA) 的核苷酸序列和转录分析
DOI：
10.1128/jb.171.2.1206-1210.1989
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发表时间：
1989
影响因子：
3.2
通讯作者：
Teruhiko Beppu
中科院分区：
文献类型：
--
作者：
S. Horinouchi;Hirokazu Suzuki;Makoto Nishiyama;Teruhiko Beppu
测定了灰色链霉菌afsa基因的核苷酸序列，该基因可能编码A因子(2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone)生物合成的关键酶。翻译起始密码子是通过寡核苷酸定向突变在适当的位置引入框外突变来鉴定的。AFSA基因编码301个氨基酸残基和32.6千道尔顿的蛋白质，其密码子使用模式与链霉菌基因具有极高的鸟嘌呤+胞嘧啶含量的一般趋势一致。高分辨率的S1核酸酶图谱表明，转录起点为ATG翻译起始密码子的第一个位置A残基。
The nucleotide sequence of the Streptomyces griseus afsA gene, possibly encoding a key enzyme for A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) biosynthesis, was determined. The translational initiation codon was identified by introducing out-of-frame mutations at appropriate positions by oligonucleotide-directed mutagenesis. The afsA gene was thus found to code for a protein of 301 amino acid residues and 32.6 kilodaltons whose codon usage pattern was in agreement with the general tendency of Streptomyces genes with an extremely high guanine-plus-cytosine content. High-resolution S1 nuclease mapping indicated that the transcriptional start point was the A residue, the first position of the ATG translational initiation codon.