Hemolysis-free blood plasma separation
Hemolysis-free blood plasma separation
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DOI：
10.1039/c4lc00149d
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发表时间：
2014-01-01
期刊：
影响因子：
6.1
通讯作者：
Lee, Luke P.
中科院分区：
文献类型：
--
作者：
Son, Jun Ho;Lee, Sang Hun;Lee, Luke P.
溶血是指红细胞破裂并将其内容物释放到血浆中，是临床上关注的主要问题。在体外，溶血可能是由于临床试验中的错误所致；在体内，溶血可能是由各种医疗条件引起的。血浆分离通常是基于血液的临床诊断程序的第一步。然而，在血浆分离过程中，红细胞因溶血而释放的抑制剂会导致诊断试验中的问题，如灵敏度低、选择性低和结果不准确。特别是，普遍缺乏简单和可靠的血浆分离方法一直是基于微流控(POC)诊断设备的主要障碍。在这里，我们介绍了一种无溶血微流控血浆分离平台。膜过滤器位于垂直上升通道的顶部(顶部过滤结构)，以减少重力辅助细胞沉积对红细胞的堵塞。与传统的底部过滤结构血浆分离平台相比，该装置使分离的血浆体积增加了约4倍(20min后2.4MU的L血浆，人全血的红细胞压积为38%)，分离血浆中的血红蛋白浓度由于防止了红细胞溶血而降低了约90%。芯片上的血浆含有类似于90%的蛋白质和类似于芯片外离心血浆中发现的100%的核酸，证实了类似的目标分子回收效率。这种简单而坚固的片内血浆分离设备与下游检测模块集成，最终创建了样品到答案微流控POC诊断设备。
Hemolysis, involving the rupture of red blood cells (RBCs) and release of their contents into blood plasma, is a major issue of concern in clinical fields. Hemolysis in vitro can occur as a result of errors in clinical trials; in vivo, hemolysis can be caused by a variety of medical conditions. Blood plasma separation is often the first step in blood-based clinical diagnostic procedures. However, inhibitors released from RBCs due to hemolysis during plasma separation can lead to problems in diagnostic tests such as low sensitivity, selectivity and inaccurate results. In particular, a general lack of simple and reliable blood plasma separation methods has been a major obstacle for microfluidic-based point-of-care (POC) diagnostic devices. Here we present a hemolysis-free microfluidic blood plasma separation platform. A membrane filter was positioned on top of a vertical up-flow channel (filter-in-top configuration) to reduce clogging of RBCs by gravity-assisted cells sedimentation. With this device, separated plasma volume was increased approximately 4-fold (2.4 mu L plasma after 20 min with 38% hematocrit human whole blood), and hemoglobin concentration in separated plasma was decreased approximately 90% due to the prevention of RBCs hemolysis, when compared to conventional filter-in-bottom configuration blood plasma separation platforms. On-chip plasma contained similar to 90% of protein and similar to 100% of nucleic acids found in off-chip centrifuged plasma, confirming comparable target molecule recovery efficiency. This simple and robust on-chip blood plasma separation device integrates with downstream detection modules to ultimately create sample-to-answer microfluidic POC diagnostics devices.