PEO-like plasma polymerized tetraglyme surface interactions with leukocytes and proteins:: in vitro and in vivo studies
PEO-like plasma polymerized tetraglyme surface interactions with leukocytes and proteins:: in vitro and in vivo studies
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DOI：
10.1163/156856202320253910
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发表时间：
2002-01-01
影响因子：
3.6
通讯作者：
Horbett, TA
中科院分区：
文献类型：
--
作者：
Shen, MC;Martinson, L;Horbett, TA
聚氧化乙烯（PEO）表面减少非特异性蛋白质和细胞与植入生物材料的相互作用，并可能改善其生物相容性。通过辉光放电等离子体沉积在氟化乙烯丙烯共聚物（FEP）基底上制备了类似PEO的聚合四甘醇二甲醚表面，并在体外吸附低于10 ng/cm（2）的纤维蛋白原。在体外和体内研究了聚合的四甘醇二甲醚表面抵抗白细胞粘附的能力。将聚合的四甘醇二甲醚和FEP皮下植入小鼠中，并在1天或4周后取出。组织学分析显示，所有4周植入物周围的纤维包裹程度相似。在FEP和四甘醇二甲醚的组织-材料界面处的纤维组织中存在深色染色的威尔斯孔。扫描电镜显示，体内巨噬细胞对聚合四甘醇二甲醚的粘附远高于对FER的粘附。与肝素化全血接触2小时后，多形核白细胞（PMN）对聚合四甘醇二甲醚的粘附远高于对FEP的粘附，而血小板对聚合四甘醇二甲醚的粘附低于对FER的粘附。细胞对聚合的四甘醇二甲醚的粘附高于对FEP的粘附;然而，当细胞悬浮在热灭活血清中时，细胞对FEP的粘附高于对聚合的四甘醇二甲醚的粘附。聚合四甘醇二甲醚的表面化学在2小时血液接触后没有变化，但在植入1天后显示氮官能团，并在植入4周后轻微降解。在血液接触或植入后，FEP的表面化学没有显著变化。松散结合的蛋白质（如聚合四甘醇二甲醚上的纤维蛋白原）可能有助于PMN和巨噬细胞的粘附，并最终导致植入物周围的纤维包裹（异物反应）。
Polyethylene oxide (PEO) surfaces reduce non-specific protein and cell interactions with implanted biomaterials and may improve their biocompatibility. PEO-like polymerized tetraglyme surfaces were made by glow discharge plasma deposition onto fluorinated ethylene propylene copolymer (FEP) substrates and were shown to adsorb less than 10 ng/cm(2) of fibrinogen in vitro. The ability of the polymerized tetraglyme surfaces to resist leukocyte adhesion was studied in vitro and in vivo. Polymerized tetraglyme and FEP were implanted subcutaneously in mice and removed after I day or 4 weeks. Histological analysis showed a similar degree of fibrous encapsulation around all of the 4-week implants. Darkly stained wells were present in the fibrous tissues at the tissue-material interface of both FEP and tetraglyme. Scanning electron micrographs showed that in vivo macrophage adhesion to polymerized tetraglyme was much higher than to FER After 2-hour contact with heparinized whole blood, polymorphonuclear leukocyte (PMN) adhesion to polymerized tetraglyme was much higher than to FEP, while platelet adhesion to polymerized tetraglyme was lower than to FER When PMNs isolated from blood were suspended in 10% autologous plasma, cell adhesion to polymerized tetraglyme was higher than to FEP; however when the cells were suspended in heat inactivated serum, cell adhesion to FEP was higher than to polymerized tetraglyme. The surface chemistry of polymerized tetraglyme did not change after 2-hour blood contact, but displayed nitrogen functional groups after 1-day implantation and became slightly degraded after 4-week implantation. The surface chemistry of FEP did not change significantly after blood contact or implantation. Loosely bound proteins such as fibrinogen on polymerized tetraglyme may contribute to the adhesion of PMNs and macrophages and ultimately to fibrous encapsulation (the foreign body response) around the implants.