Lipid raft dynamics linked to sperm competency for fertilization in mice
Lipid raft dynamics linked to sperm competency for fertilization in mice
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DOI：
10.1111/gtc.12491
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发表时间：
2017-05
期刊：
影响因子：
2.1
通讯作者：
Hitomi Watanabe;Rie Takeda;K. Hirota;G. Kondoh
中科院分区：
文献类型：
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作者：
Hitomi Watanabe;Rie Takeda;K. Hirota;G. Kondoh
众所周知，哺乳动物的精子经过几个成熟过程后获得受精能力，特别是在雌性生殖道内。在之前的研究中，我们发现在精子成熟过程中，糖基磷脂酰肌醇(GPI)锚定蛋白(GPI-AP)的释放和脂筏的运动都发生了。在一些遗传学研究中，GPI-AP的释放是小鼠精子受精能力的关键步骤。在这里，我们通过比较两个近交系小鼠C57BL/6和BALB/c的精子成熟过程表明，脂筏运动也是精子受精的基础。我们发现，与C57BL/6相比，BALB/c的精子成熟过程中神经节苷脂GM1的运动唯一减少，以及其他被检测的精子成熟参数，如GPI-AP释放，精子向输卵管的迁移，胆固醇外流，蛋白酪氨酸磷酸化和顶体反应，并与精子生育表型密切相关。GM1运动与体外受精能力的关系在其他品系的小鼠中得到了证实，提示脂筏运动是完成精子成熟过程的重要步骤之一。
It is well known that mammalian sperm acquires fertilization ability after several maturation processes, particularly within the female reproductive tract. In a previous study, we found that both glycosylphosphatidylinositol (GPI)‐anchored protein (GPI‐AP) release and lipid raft movement occur during the sperm maturation process. In several genetic studies, release of GPI‐AP is a crucial step for sperm fertilization ability in the mouse. Here, we show that lipid raft movement is also fundamental for sperm to be competent for fertilization by comparing the sperm maturation process of two mouse inbred strains, C57BL/6 and BALB/c. We found that ganglioside GM1 movement was exclusively reduced in BALB/c compared with C57BL/6 among other examined sperm maturation parameters, such as GPI‐AP release, sperm migration to the oviduct, cholesterol efflux, protein tyrosine phosphorylation and acrosome reaction, and was strongly linked to sperm fertility phenotype. The relationship between GM1 movement and in vitro fertilization ability was confirmed in other mouse strains, suggesting that lipid raft movement is one of the important steps for completing the sperm maturation process.