Single-Molecule Biophysical Techniques to Study Actomyosin Force Transduction.
Single-Molecule Biophysical Techniques to Study Actomyosin Force Transduction.
复制标题
研究肌动球蛋白力传导的单分子生物物理技术。
DOI：
10.1007/978-3-030-38062-5_6
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发表时间：
2020
影响因子：
--
通讯作者：
Fineberg,Adam
中科院分区：
文献类型：
--
作者：
Takagi,Yasuharu;Hundt,Nikolas;Fineberg,Adam
在细胞环境中，分子马达可以协同工作，以进行各种重要的生理功能和过程，这些功能和过程对细胞的生存至关重要。然而，为了破译这些分子马达如何工作的机制，单分子显微镜技术已经成为了解这些马达蛋白新兴的整体行为的分子基础的流行方法。在本章中，我们将讨论各种单分子生物物理成像技术，这些技术已被用于揭示肌球蛋白的力学和动力学。本章应被视为对许多现有技术的一般概述和介绍性指南;然而，由于其他章节将更彻底地讨论其中的一些技术，读者应该参考这些章节以获得进一步的细节和讨论。特别是，我们将专注于基于散射的单分子显微镜方法，其中一些方法近年来变得越来越流行，我们实验室的工作一直围绕这些方法展开。
Inside the cellular environment, molecular motors can work in concert to conduct a variety of important physiological functions and processes that are vital for the survival of a cell. However, in order to decipher the mechanism of how these molecular motors work,single-molecule microscopy techniqueshave been popular methods to understand the molecular basis of the emerging ensemble behavior of these motor proteins.In this chapter, we discuss various single-molecule biophysical imaging techniques that have been used to expose the mechanics and kinetics of myosins. The chapter should be taken as a general overview and introductory guide to the many existing techniques; however, since other chapters will discuss some of these techniques more thoroughly, the readership should refer to those chapters for further details and discussions. In particular, we will focus on scattering-based single-molecule microscopy methods, some of which have become more popular in the recent years and around which the work in our laboratories has been centered.