Studying Dynamic Plasma Membrane Binding of TCR-CD3 Chains During Immunological Synapse Formation Using Donor-Quenching FRET and FLIM-FRET
Studying Dynamic Plasma Membrane Binding of TCR-CD3 Chains During Immunological Synapse Formation Using Donor-Quenching FRET and FLIM-FRET
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DOI：
10.1007/978-1-4939-6881-7_16
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发表时间：
2017-01-01
期刊：
影响因子：
--
通讯作者：
Wucherpfennig, Kai W.
中科院分区：
文献类型：
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作者：
Gagnon, Etienne;Connolly, Audrey;Wucherpfennig, Kai W.
在过去的十年中，显微镜技术的时间和空间分辨率的进步使得能够解剖参与T细胞免疫突触（IS）形成的分子事件。使用福斯特共振能量转移（FRET）和荧光寿命成像显微镜（FLIM）的组合，我们已经证明了动态质膜结合的T细胞受体（TCR）相关的CD3链和其他T细胞跨膜受体的胞质结构域。我们已经开发出在稳态和在IS的受体触发期间成像这种膜结合的方法。细胞质结构域的质膜结合可能代表了调节免疫系统中重要受体的信号传导功能的新机制。
Over the last decade, advancements in the time and space resolution of microscopy technologies have enabled dissection of the molecular events involved in T cell Immunological Synapse (IS) formation. Using a combination of Forster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imagining Microscopy (FLIM), we have demonstrated dynamic plasma membrane binding by cytoplasmic domains of T cell receptor (TCR)-associated CD3 chains and other T cell transmembrane receptors. We have developed methods for imaging such membrane binding both at steady state and during receptor triggering at the IS. Plasma membrane binding by cytoplasmic domains may represent a novel mechanism for regulating the signaling function of important receptors in the immune system.